ArticleJundishapur journal of microbiology2015
Detection of Staphylococcus Enterotoxin B (SEB) Using an Immunochromatographic Test Strip.
Article in Jundishapur journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 28 citations in OpenAlex.
- Ultrasensitive Staphylococcal Enterotoxin B Quantum Dot-Based Lateral Flow Immunoassays for Rapid Testing of Poultry Rinsates and Milk.Journal of fluorescence · 2025Article
- Artificial intelligence enhanced electrochemical immunoassay for staphylococcal enterotoxin B.Scientific reports · 2025Article
- Recent Advances in Aptamer-Based Biosensors for Bacterial Detection.Biosensors · 2024Review
- Occurrence and Patterns of Enterotoxin Genes,Microorganisms · 2023Article
- Antibiogram and phylogenetic diversity of enterotoxigenicSaudi journal of biological sciences · 2020Article
- Rapid Detection of Staphylococcal Enterotoxin-B by Lateral Flow Assay.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2019Article
- Enterotoxigenicity and Antimicrobial Resistance ofFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training.
objectivesIn our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity. MATERIALS AND
methodsThe strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences.
resultsIn total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay.
conclusionsThe sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration.
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Registered trials
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