ArticleJournal of tropical pediatrics2016
The Role of 16S rRNA Gene Sequencing in Confirmation of Suspected Neonatal Sepsis.
Article in Journal of tropical pediatrics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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The trial behind it
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 22 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- The Accuracy of 16S rRNA Polymerase Chain Reaction for the Diagnosis of Neonatal Sepsis: A Meta-Analysis.BioMed research international · 2021Pooled it
- Comparison of metagenomic next-generation sequencing and blood culture for diagnosis of bloodstream infections.Frontiers in cellular and infection microbiology · 2024Article
- Molecular Methodologies for Improved Polymicrobial Sepsis Diagnosis.International journal of molecular sciences · 2022Review
- Machine Learning Approaches to Predict In-Hospital Mortality among Neonates with Clinically Suspected Sepsis in the Neonatal Intensive Care Unit.Journal of personalized medicine · 2021Article
- Tracking bacterial DNA patterns in septic progression using 16s rRNA gene amplicon sequencing analysis.International journal of clinical and experimental pathology · 2021Article
- Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis.The Journal of international medical research · 2020Article
- Identification of potential genes and miRNAs associated with sepsis based on microarray analysis.Molecular medicine reports · 2018Article
- Diagnostics for neonatal sepsis: current approaches and future directions.Pediatric research · 2017Review
- Analysis of early-onset bloodstream infection due to Escherichia coli infection in premature babies.Medicine · 2017Observational
- Assessing the diagnostic potential of 16SrRNA gene for neonatal sepsis: A tertiary care hospital study in South India.Medical journal, Armed Forces IndiaArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different molecular assays for the detection of bacterial DNA in the peripheral blood represented a diagnostic tool for neonatal sepsis. We targeted to evaluate the role of 16S rRNA gene sequencing to screen for bacteremia to confirm suspected neonatal sepsis (NS) and compare with risk factors and septic screen testing. Sixty-two neonates with suspected NS were enrolled. White blood cells count, I/T ratio, C-reactive protein, blood culture and 16S rRNA sequencing were performed. Blood culture was positive in 26% of cases, and PCR was positive in 26% of cases. Evaluation of PCR for the diagnosis of NS showed sensitivity 62.5%, specificity 86.9%, PPV 62.5%, NPV 86.9% and accuracy of 79.7%. 16S rRNA PCR increased the sensitivity of detecting bacterial DNA in newborns with signs of sepsis from 26 to 35.4%, and its use can be limited to cases with the most significant risk factors and positive septic screen.
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Registered trials
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