ArticleDental materials : official publication of the Academy of Dental Materials2015
Cytotoxicity and osteogenic potential of silicate calcium cements as potential protective materials for pulpal revascularization.
Article in Dental materials : official publication of the Academy of Dental Materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
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Who cites it
44 citing papers in PubMed.
- Direct pulp capping in primary molars using a resin-modified Portland cement-based material (TheraCal) compared to MTA with 12-month follow-up: a randomised clinical trial.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2018Trial
- Clinical and Radiographic Outcomes of Vital Pulp Therapy Using Resin-Modified Versus Conventional Calcium Silicate-Based Materials: A Systematic Review and Meta-Analysis.Journal of functional biomaterials · 2026Review
- In vivo long-term effects of bioactive mineral trioxide aggregate on the inflammatory response and repair in dental pulp.Brazilian oral research · 2026Article
- Cytocompatibility of Three Calcium Silicate-Based Biomaterials: A Systematic Review and Meta-Analysis.Iranian endodontic journal · 2026Review
- Responses of stem cells isolated from human primary teeth to calcium-containing pulp capping materials.BMC oral health · 2025Article
- Effect of light-cured pulp capping materials on human dental pulp cells in vitro.International endodontic journal · 2025Article
- Review
- Effect of nanoparticulate CaCOScientific reports · 2025Article
- Biocompatibility and Cytotoxicity of Pulp-Capping Materials on DPSCs, With Marker mRNA Expressions.International dental journal · 2024Article
- Evaluation of Antimicrobial Properties, Cell Viability, and Metalloproteinase Activity of Bioceramic Endodontic Materials Used in Vital Pulp Therapy.Journal of functional biomaterials · 2024Article
- The solubility, pH value, chemical structure, radiopacity, and cytotoxicity of four different root canal sealers: an in vitro study.Clinical oral investigations · 2023Article
- Comparative Biocompatibility and Odonto-/Osteogenesis Effects of Hydraulic Calcium Silicate-Based Cements in Simulated Direct and Indirect Approaches for Regenerative Endodontic Treatments: A Systematic Review.Journal of functional biomaterials · 2023Review
- Comparative chemical properties, bioactivity, and cytotoxicity of resin-modified calcium silicate-based pulp capping materials on human dental pulp stem cells.Clinical oral investigations · 2022Article
- Bond Strength of Adhesive Systems to Calcium Silicate-Based Materials: A Systematic Review and Meta-Analysis of In Vitro Studies.Gels (Basel, Switzerland) · 2022Review
- Article
- Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells.Materials (Basel, Switzerland) · 2021Article
- Article
- Biological Characteristics and Odontogenic Differentiation Effects of Calcium Silicate-Based Pulp Capping Materials.Materials (Basel, Switzerland) · 2021Article
- Cytocompatibility and bioactive properties of the new dual-curing resin-modified calcium silicate-based material for vital pulp therapy.Clinical oral investigations · 2021Article
- Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
objectivesIn pulpal revascularization, a protective material is placed coronal to the blood clot to prevent recontamination and to facilitate osteogenic differentiation of mesenchymal stem cells to produce new dental tissues. Although mineral trioxide aggregate (MTA) has been the material of choice for clot protection, it is easily displaced into the clot during condensation. The present study evaluated the effects of recently introduced calcium silicate cements (Biodentine and TheraCal LC) on the viability and osteogenic differentiation of human dental pulp stem cells (hDPSCs) by comparing with MTA Angelus.
methodsCell viability was assessed using XTT assay and flow cytometry. The osteogenic potential of hDPSCs exposed to calcium silicate cements was examined using qRT-PCR for osteogenic gene expressions, alkaline phosphatase enzyme activity, Alizarin red S staining and transmission electron microscopy of extracellular calcium deposits. Parametric statistical methods were employed for analyses of significant difference among groups, with α=0.05.
resultsThe cytotoxic effects of Biodentine and TheraCal LC on hDPSCs were time- and concentration-dependent. Osteogenic differentiation of hDPSCs was enhanced after exposure to Biodentine that was depleted of its cytotoxic components. This effect was less readily observed in hDPSCs exposed to TheraCal LC, although both cements supported extracellular mineralization better than the positive control (zinc oxide-eugenol-based cement). SIGNIFICANCE: A favorable tissue response is anticipated to occur with the use of Biodentine as a blood clot-protecting material for pulpal revascularization. Further investigations with the use of in vivo animal models are required to validate the potential adverse biological effects of TheraCal LC on hDPSCs.
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