ArticleAnalytical chemistry2015
Photolithographic Synthesis of High-Density DNA and RNA Arrays on Flexible, Transparent, and Easily Subdivided Plastic Substrates.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- A wrinkled structure of gold film greatly improves the signaling of electrochemical aptamer-based biosensors.RSC advances · 2020Article
- Gold Nanopost-Shell Arrays Fabricated by Nanoimprint Lithography as a Flexible Plasmonic Sensing Platform.Nanomaterials (Basel, Switzerland) · 2019Article
- Generation of small molecule-binding RNA arrays and their application to fluorogen-binding RNA aptamers.Methods (San Diego, Calif.) · 2019Article
- Encrypted Oligonucleotide Arrays for Molecular Authentication.ACS combinatorial science · 2019Article
- Lab-on-a-Film disposable for genotyping multidrug-resistant Mycobacterium tuberculosis from sputum extracts.Lab on a chip · 2019Article
- An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer.Scientific reports · 2019Article
- Spotting, Transcription andComputational and structural biotechnology journal · 2019Review
- High-Density RNA Microarrays Synthesized In Situ by Photolithography.Angewandte Chemie (International ed. in English) · 2018Article
- High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions.Nucleic acids research · 2018Article
- Parallel DNA Synthesis on Poly(ethylene terephthalate).Chembiochem : a European journal of chemical biology · 2017Article
- Layer-by-Layer Assembly of Amine-Reactive Multilayers Using an Azlactone-Functionalized Polymer and Small-Molecule Diamine Linkers.Biomacromolecules · 2017Article
- Mapping the affinity landscape of Thrombin-binding aptamers on 2΄F-ANA/DNA chimeric G-Quadruplex microarrays.Nucleic acids research · 2017Article
- Flexible Substrate-Based Devices for Point-of-Care Diagnostics.Trends in biotechnology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The photolithographic fabrication of high-density DNA and RNA arrays on flexible and transparent plastic substrates is reported. The substrates are thin sheets of poly(ethylene terephthalate) (PET) coated with cross-linked polymer multilayers that present hydroxyl groups suitable for conventional phosphoramidite-based nucleic acid synthesis. We demonstrate that by modifying array synthesis procedures to accommodate the physical and chemical properties of these materials, it is possible to synthesize plastic-backed oligonucleotide arrays with feature sizes as small as 14 μm × 14 μm and feature densities in excess of 125 000/cm(2), similar to specifications attainable using rigid substrates such as glass or glassy carbon. These plastic-backed arrays are tolerant to a wide range of hybridization temperatures, and improved synthetic procedures are described that enable the fabrication of arrays with sequences up to 50 nucleotides in length. These arrays hybridize with S/N ratios comparable to those fabricated on otherwise identical arrays prepared on glass or glassy carbon. This platform supports the enzymatic synthesis of RNA arrays and proof-of-concept experiments are presented showing that the arrays can be readily subdivided into smaller arrays (or "millichips") using common laboratory-scale laser cutting tools. These results expand the utility of oligonucleotide arrays fabricated on plastic substrates and open the door to new applications for these important bioanalytical tools.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.