ArticleBMC genomics2015
Novel method to detect microRNAs using chip-based QuantStudio 3D digital PCR.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 74 citations in OpenAlex.
- Review
- Rapid Manufacturing of Glass-Based Digital Nucleic Acid Amplification Chips by Ultrafast Bessel Pulses.Small science · 2024Article
- SPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testing.Microsystems & nanoengineering · 2024Article
- Circulating Nucleic Acids in Colorectal Cancer: Diagnostic and Prognostic Value.Disease markers · 2024Review
- Novel insights on perils and promises of miRNA in understanding colon cancer metastasis and progression.Medical oncology (Northwood, London, England) · 2023Review
- A Chamber-Based Digital PCR Based on a Microfluidic Chip for the Absolute Quantification and Analysis of KRAS Mutation.Biosensors · 2023Article
- Article
- Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR.Biomedicines · 2022Article
- Perspectives in translating microfluidic devices from laboratory prototyping into scale-up production.Biomicrofluidics · 2022Article
- MiR-486-5p Targets CD133+ Lung Cancer Stem Cells through the p85/AKT Pathway.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Experimental MicroRNA Detection Methods.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Real-time digital polymerase chain reaction (PCR) as a novel technology improves limit of detection for rare allele assays.Translational lung cancer research · 2021Article
- Circulating extracellular vesicle-encapsulated microRNA as screening biomarkers for intraductal papillary mucinous neoplasm.Oncology letters · 2020Article
- High filling rate digital PCR through-hole array chip with double independent S-shaped flow channels.Biomicrofluidics · 2020Article
- miR-3940-5p/miR-8069 ratio in urine exosomes is a novel diagnostic biomarker for pancreatic ductal adenocarcinoma.Oncology letters · 2020Article
- Isothermal digital detection of microRNAs using background-free molecular circuit.Science advances · 2020Article
- A microfluidic alternating-pull-push active digitization method for sample-loss-free digital PCR.Lab on a chip · 2019Article
- c-Myc shuttled by tumour-derived extracellular vesicles promotes lung bronchial cell proliferation through miR-19b and miR-92a.Cell death & disease · 2019Article
- Analysis of circulating non-coding RNAs in a non-invasive and cost-effective manner.Trends in analytical chemistry : TRAC · 2019Article
- Slip-driven microfluidic devices for nucleic acid analysis.Biomicrofluidics · 2019Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundResearch efforts for the management of cancer, in particular for lung cancer, are directed to identify new strategies for its early detection. MicroRNAs (miRNAs) are a new promising class of circulating biomarkers for cancer detection, but lack of consensus on data normalization methods has affected the diagnostic potential of circulating miRNAs. There is a growing interest in techniques that allow an absolute quantification of miRNAs which could be useful for early diagnosis. Recently, digital PCR, mainly based on droplets generation, emerged as an affordable technology for precise and absolute quantification of nucleic acids.
resultsIn this work, we described a new interesting approach for profiling circulating miRNAs in plasma samples using a chip-based platform, the QuantStudio 3D digital PCR. The proposed method was validated using synthethic oligonucleotide at serial dilutions in plasma samples of lung cancer patients and in lung tissues and cell lines.
conclusionGiven its reproducibility and reliability, our approach could be potentially applied for the identification and quantification of miRNAs in other biological samples such as circulating exosomes or protein complexes. As chip-digital PCR becomes more established, it would be a robust tool for quantitative assessment of miRNA copy number for diagnosis of lung cancer and other diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.