ArticleDevelopment (Cambridge, England)2015
ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 315 papers.
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Who cites it
315 citing papers in PubMed.
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- Time-dependent bistability leads to critical slowing down during floral transition in Arabidopsis.Nature communications · 2026Article
- Single-Cell and Spatial Omics Technologies in Rice Abiotic Stress Biology: A Methodological Review.International journal of molecular sciences · 2026Review
- A simple hand-sectioning approach for cellular-resolution imaging of anatomy and gene expression in small developing organs and boundary regions in Arabidopsis.Plant methods · 2026Article
- Rational design of T-DNA vectors enables predictable, single-copy integration inbioRxiv : the preprint server for biology · 2026Article
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- Brassinosteroid signaling regulates shoot apical meristem homeostasis via orchestrating cytokinin and WUSCHEL activity.Science advances · 2026Article
- 3D imaging reveals robustness and plasticity of cell division in rice early embryogenesis.Plant & cell physiology · 2026Article
- Evolutionary diversity of cell-type-specific expression and stress response in Brassicaceae roots.Nature communications · 2026Article
- Stem cell activity is linked to a transient acquisition of pluripotency during callus proliferation.Nature communications · 2026Article
- AGP and EXO-LIKE genes promote brassinosteroid-dependent anisotropic growth.The New phytologist · 2026Article
- Imaging and genetic toolbox to study Arabidopsis embryogenesis.The New phytologist · 2026Review
- Evolutionary diversity of cell-type-specific expression and stress response in Brassicaceae roots.bioRxiv : the preprint server for biology · 2026Article
- NUTCRACKER orchestrates cortical cell divisions and sustains stem cell niche integrity in the rice root meristem.Journal of experimental botany · 2026Article
- Grafting reveals organ-autonomous and feedback roles of root phloem development in source-sink dynamics.Journal of experimental botany · 2026Article
- In perennial Arabis alpina, CONSTANS and FLOWERING LOCUS T have common and distinct effects on flowering and inflorescence architecture.The New phytologist · 2026Article
- Choosing a skull clearing technique for chronic mesoscopic optical imaging in awake mice.Neurophotonics · 2026Article
255 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
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Abstract
Imaging techniques for visualizing and analyzing precise morphology and gene expression patterns are essential for understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a clearing method using chemical solutions, termed ClearSee, for deep imaging of morphology and gene expression in plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting the refractive index mismatch, whole-organ and whole-plant imaging can be performed by both confocal and two-photon excitation microscopy in ClearSee-treated samples. Moreover, ClearSee is applicable to multicolor imaging of fluorescent proteins to allow structural analysis of multiple gene expression. Given that ClearSee is compatible with staining by chemical dyes, the technique is useful for deep imaging in conjunction with genetic markers and for plant species not amenable to transgenic approaches. This method is useful for whole imaging for intact morphology and will help to accelerate the discovery of new phenomena in plant biological research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.