Evidence map›Paper›PMID 26493380›Full record

ArticleChembiochem : a European journal of chemical biology2015

Comparison of 10,11-Dehydrocurvularin Polyketide Synthases from Alternaria cinerariae and Aspergillus terreus Highlights Key Structural Motifs.

Rachel V K Cochrane, Zhizeng Gao, Gareth R Lambkin, Wei Xu, Jaclyn M Winter, Sandra L Marcus, Yi Tang, John C Vederas

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
  3. Biosynthesis of Cytosporones in Leotiomycetous Filamentous Fungi.Journal of the American Chemical Society · 2024
    Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Rachel V K CochraneDepartment of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Zhizeng GaoDepartment of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Gareth R LambkinDepartment of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Wei XuDepartment of Chemical and Biomolecular Engineering and, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
Jaclyn M WinterDepartment of Medicinal Chemistry, The University of Utah, Salt Lake City, Utah, 84112, USA.
Sandra L MarcusDepartment of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
Yi TangDepartment of Chemical and Biomolecular Engineering and, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
John C VederasDepartment of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada. john.vederas@ualberta.ca.
University of Alberta · CAUniversity of California, Los Angeles · USUniversity of Utah · US

Funding

Rediscovering Natural Chemical Diversity -1DP1GM106413DP1GM106413 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TANG, YI · 2012 to 2016
$3.9M
Characterization and Engineering of Fungal Megasynthases in Escherichia coliR01GM085128 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TANG, YI · 2008 to 2016
$2.1M
NIGMS NIH HHS DP1 GM106413NIGMS NIH HHS GM085128NIGMS NIH HHS GM106413NIGMS NIH HHS R01 GM085128
6 · The paper itself

Abstract

Iterative type I polyketide synthases (PKSs) from fungi are multifunctional enzymes that use their active sites repeatedly in a highly ordered sequence to assemble complex natural products. A phytotoxic macrolide with anticancer properties, 10,11-dehydrocurvularin (DHC), is produced by cooperation of a highly reducing (HR) iterative PKS and a non-reducing (NR) iterative PKS. We have identified the DHC gene cluster in Alternaria cinerariae, heterologously expressed the active HR PKS (Dhc3) and NR PKS (Dhc5) in yeast, and compared them to corresponding proteins that make DHC in Aspergillus terreus. Phylogenetic analysis and homology modeling of these enzymes identified variable surfaces and conserved motifs that are implicated in product formation.

Indexed as

AlternariaAspergillusCatalytic DomainMultigene FamilyPhylogenyPolyketide SynthasesProtein Structure, TertiaryZearalenonedehydrocurvularinPolyketide SynthasesZearalenonebioinformaticsbiosynthesisdehydrocurvularinheterologous expressionpolyketide

Identifiers

PMID26493380
PMCPMC4804156
OpenAlexW2147532559

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.