ArticleJournal of nanobiotechnology2015
Glucose availability determines silver nanoparticles toxicity in HepG2.
Article in Journal of nanobiotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Synthesis, Cytotoxic and Genotoxic Evaluation of Drug-Loaded Silver Nanoparticles with Mebeverine and Its Analog.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Unveiling the cytotoxic and anti-proliferative potential of green-synthesized silver nanoparticles mediated byRSC advances · 2024Article
- Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles.Heliyon · 2023Article
- How to Use Respiratory Chain Inhibitors in Toxicology Studies-Whole-Cell Measurements.International journal of molecular sciences · 2022Article
- Nanoparticle Effects on Stress Response Pathways and Nanoparticle-Protein Interactions.International journal of molecular sciences · 2022Review
- Review
- Silver Nanoparticles Modulate the Epithelial-to-Mesenchymal Transition in Estrogen-Dependent Breast Cancer Cells In Vitro.International journal of molecular sciences · 2021Article
- Products of Lipid Peroxidation as a Factor in the Toxic Effect of Silver Nanoparticles.Materials (Basel, Switzerland) · 2020Article
- Susceptibility of HepG2 Cells to Silver Nanoparticles in Combination with other Metal/Metal Oxide Nanoparticles.Materials (Basel, Switzerland) · 2020Article
- Silver nanoparticles: Synthesis, medical applications and biosafety.Theranostics · 2020Review
- Bactericidal and Cytotoxic Properties of Silver Nanoparticles.International journal of molecular sciences · 2019Review
- Silver Nanoparticles: Synthetic Routes, In Vitro Toxicity and Theranostic Applications for Cancer Disease.Nanomaterials (Basel, Switzerland) · 2018Review
- Silver nanoparticles can attenuate nitrative stress.Redox biology · 2017Article
- Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment.International journal of nanomedicine · 2017Article
- Silver Nanoparticles Induce HePG-2 Cells Apoptosis Through ROS-Mediated Signaling Pathways.Nanoscale research letters · 2016Article
- Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches.International journal of molecular sciences · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe increasing body of evidence suggest that nanomaterials toxicity is associated with generation of oxidative stress. In this paper we investigated the role of respiration in silver nanoparticles (AgNPs) generated oxidative stress and toxicity. Since cancer cells rely on glucose as the main source of energy supply, glucose availability might be an important determinant of NPs toxicity.
methodsAgNPs of 20 nm nominal diameter were used as a model NPs. HepG2 cells were cultured in the media with high (25 mM) or low (5.5 mM) glucose content and treated with 20 nm AgNPs. AgNPs-induced toxicity was tested by neutral red assay. Generation of H2O2 in mitochondria was evaluated by use of mitochondria specific protein indicator HyPer-Mito. Expression of a 77 oxidative stress related genes was assessed by qPCR. The activity of antioxidant enzymes was estimated colorimetrically by dedicated methods in cell homogenates.
resultsAgNPs-induced dose-dependent generation of H2O2 and toxicity was observed. Toxicity of AgNPs towards cells maintained in the low glucose medium was significantly lower than the toxicity towards cells growing in the high glucose concentration. Scarceness of glucose supply resulted in upregulation of the endogenous antioxidant defence mechanisms that in turn alleviated AgNPs dependent ROS generation and toxicity.
conclusionGlucose availability can modify toxicity of AgNPs via elevation of antioxidant defence triggered by oxidative stress resulted from enhanced oxidative phosphorylation in mitochondria and associated generation of ROS. Presented results strengthen the idea of strong linkage between NPs toxicity and intracellular respiration and possibly other mitochondria dependent processes.
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