ArticleScientific reports2015
Identification of a phosphinothricin-resistant mutant of rice glutamine synthetase using DNA shuffling.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 25 citations in OpenAlex.
- Analysis of glutamine synthetase target-site mutations and their role in endowing glufosinate-ammonium resistance.Pest management science · 2026Article
- Genome-wide association study reveals the genetic basis of rice resistance to three herbicides.Frontiers in plant science · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Adapting enzymes to improve their functionality in plants: why and how.Biochemical Society transactions · 2023Review
- Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirement.Nature communications · 2023Article
- A naturally evolved mutation (Ser59Gly) in glutamine synthetase confers glufosinate resistance in plants.Journal of experimental botany · 2022Article
- Genetic Diversity in Nitrogen Fertiliser Responses and N Gas Emission in Modern Wheat.Frontiers in plant science · 2022Article
- Mechanistic Basis for ATP-Dependent Inhibition of Glutamine Synthetase by Tabtoxinine-β-lactam.Biochemistry · 2018Article
- Concurrent Overexpression ofFrontiers in plant science · 2018Article
- Arg156 in the AP2-Domain Exhibits the Highest Binding Activity among the 20 Individuals to the GCC Box in BnaERF-B3-hy15, a Mutant ERF Transcription Factor fromFrontiers in plant science · 2016Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To date, only bar/pat gene derived from Streptomyces has been used to generate the commercial PPT-resistant crops currently available in the market. The limited source of bar/pat gene is probably what has caused the decrease in PPT-tolerance, which has become the main concern of those involved in field management programs. Although glutamine synthetase (GS) is the target enzyme of PPT, little study has been reported about engineering PPT-resistant plants with GS gene. Then, the plant-optimized GS gene from Oryza sativa (OsGS1S) was chemically synthesized in the present study by PTDS to identify a GS gene for developing PPT-tolerant plants. However, OsGS1S cannot be directly used for developing PPT-tolerant plants because of its poor PPT-resistance. Thus, we performed DNA shuffling on OsGS1S, and one highly PPT-resistant mutant with mutations in four amino acids (A63E, V193A, T293A and R295K) was isolated after three rounds of DNA shuffling and screening. Among the four amino acids substitutions, only R295K was identified as essential in altering PPT resistance. The R295K mutation has also never been previously reported as an important residue for PPT resistance. Furthermore, the mutant gene has been transformed into Saccharomyces cerevisiae and Arabidopsis to confirm its potential in developing PPT-resistant crops.
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