ReviewInternational journal of molecular sciences2015
High-Throughput Screening in Protein Engineering: Recent Advances and Future Perspectives.
Review in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- StabLyzeGraph: High-throughput screening of combinatorial mutations using graph neural networks.Protein science : a publication of the Protein Society · 2026Article
- High-throughput screening for optimizing adoptive T cell therapies.Experimental hematology & oncology · 2024Review
- GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.Protein science : a publication of the Protein Society · 2024Article
- Integrating Computational Design and Experimental Approaches for Next-Generation Biologics.Biomolecules · 2024Review
- Semirational engineering ofApplied and environmental microbiology · 2023Article
- Fundamental concepts of protein therapeutics and spacing in oncology: an updated comprehensive review.Medical oncology (Northwood, London, England) · 2023Review
- Transient binding sites at the surface of haloalkane dehalogenase LinB as locations for fine-tuning enzymatic activity.PloS one · 2023Article
- Applying molecular and phenotypic screening assays to identify efficient quorum quenching lactonases.Enzyme and microbial technology · 2022Article
- Strategies for Improving Small-Molecule Biosensors in Bacteria.Biosensors · 2022Review
- Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning.Scientific reports · 2021Article
- A systematic analysis of the beta hairpin motif in the Protein Data Bank.Protein science : a publication of the Protein Society · 2021Article
- Stable and selective permeable hydrogel microcapsules for high-throughput cell cultivation and enzymatic analysis.Microbial cell factories · 2020Article
- Review
- High-throughput droplet-based microfluidics for directed evolution of enzymes.Electrophoresis · 2019Review
- High-Throughput Approaches in Carbohydrate-Active Enzymology: Glycosidase and Glycosyl Transferase Inhibitors, Evolution, and Discovery.Angewandte Chemie (International ed. in English) · 2019Review
- Natural selection in compartmentalized environment with reshuffling.Journal of mathematical biology · 2019Article
- Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology.Micromachines · 2019Review
- The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering.Chembiochem : a European journal of chemical biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the last three decades, protein engineering has established itself as an important tool for the development of enzymes and (therapeutic) proteins with improved characteristics. New mutagenesis techniques and computational design tools have greatly aided in the advancement of protein engineering. Yet, one of the pivotal components to further advance protein engineering strategies is the high-throughput screening of variants. Compartmentalization is one of the key features allowing miniaturization and acceleration of screening. This review focuses on novel screening technologies applied in protein engineering, highlighting flow cytometry- and microfluidics-based platforms.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.