ArticleACS chemical biology2016
Self-Assembling NanoLuc Luciferase Fragments as Probes for Protein Aggregation in Living Cells.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Rapid, expression-free bacteriophage-based specific detection of target bacteria by conditional release of encapsidated reporter molecules.Bioengineering & translational medicine · 2026Article
- SplitSeek-Pro: accurate prediction of splittable sites on protein structures.Nature communications · 2026Article
- Mutational Scanning of α-Synuclein using a Clickable Protein Tag Reveals Determinants of Membrane-Induced Aggregation.bioRxiv : the preprint server for biology · 2026Article
- Real-time imaging of protein microenvironment changes in cells with rotor-based fluorescent amino acids.Nature chemical biology · 2026Article
- DNA Origami-Based Luminescent Biosensors Enabling Smartphone Detection of Nucleic Acid Sequences.ACS omega · 2025Article
- A putative design for the electromagnetic activation of split proteins for molecular and cellular manipulation.Frontiers in bioengineering and biotechnology · 2024Article
- Article
- Illuminating cellular and extracellular vesicle-mediated communication via a split-Nanoluc reporterCell reports methods · 2023Article
- Bioluminescence Production by Turnip Yellows Virus Infectious Clones: A New Way to Monitor Plant Virus Infection.International journal of molecular sciences · 2022Article
- Generalized Bioluminescent Platform To Observe and Track Cellular Interactions.Bioconjugate chemistry · 2022Article
- Article
- Direct visualization and profiling of protein misfolding and aggregation in live cells.Current opinion in chemical biology · 2021Review
- AggFluor: Fluorogenic Toolbox Enables Direct Visualization of the Multi-Step Protein Aggregation Process in Live Cells.Journal of the American Chemical Society · 2020Article
- DiB-splits: nature-guided design of a novel fluorescent labeling split system.Scientific reports · 2020Article
- A Luminescence-Based System for Identification of Genetically Encodable Inhibitors of Protein Aggregation.ACS omega · 2020Article
- A luminescence-based assay for monitoring changes in alpha-synuclein aggregation in living cells.RSC advances · 2020Article
- Dynamic monitoring of STAT3 activation in live cells using a novel STAT3 Phospho-BRET sensor.American journal of nuclear medicine and molecular imaging · 2019Article
- Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells.Methods in enzymology · 2019Article
- Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior.Molecular cell · 2018Article
- A Review of Meningococcal Disease and Vaccination Recommendations for Travelers.Infectious diseases and therapy · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Given the clear role of protein aggregation in human disease, there is a critical need for assays capable of quantifying protein aggregation in living systems. We hypothesized that the inherently low background and biocompatibility of luminescence signal readouts could provide a potential solution to this problem. Herein, we describe a set of self-assembling NanoLuc luciferase (Nluc) fragments that produce a tunable luminescence readout that is dependent upon the solubility of a target protein fused to the N-terminal Nluc fragment. To demonstrate this approach, we employed this assay in bacteria to assess mutations known to disrupt amyloid-beta (Aβ) aggregation as well as disease-relevant mutations associated with familial Alzheimer's diseases. The luminescence signal from these experiments correlates with the reported aggregation potential of these Aβ mutants and reinforces the increased aggregation potential of disease-relevant mutations in Aβ1-42. To further demonstrate the utility of this approach, we show that the effect of small molecule inhibitors on Aβ aggregation can be monitored using this system. In addition, we demonstrate that aggregation assays can be ported into mammalian cells. Taken together, these results indicate that this platform could be used to rapidly screen for mutations that influence protein aggregation as well as inhibitors of protein aggregation. This method offers a novel, genetically encodable luminescence readout of protein aggregation in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.