ArticlePLoS genetics2015
MET18 Connects the Cytosolic Iron-Sulfur Cluster Assembly Pathway to Active DNA Demethylation in Arabidopsis.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- The Cytosolic Iron-Sulfur Cluster Assembly Targeting Complex Is Hijacked by Magnaporthe Effector AvrPi9 to Suppress Rice Resistance.Plant biotechnology journal · 2026Article
- DNA methylation either antagonizes or promotes Polycomb recruitment at transposable elements in Arabidopsis.Nature communications · 2026Article
- Article
- Redox-Epigenetic Crosstalk in Plant Stress Responses: The Roles of Reactive Oxygen and Nitrogen Species in Modulating Chromatin Dynamics.International journal of molecular sciences · 2025Review
- Control of DNA demethylation by superoxide anion in plant stem cells.Nature chemical biology · 2025Article
- Beyond NPK: Mineral Nutrient-Mediated Modulation in Orchestrating Flowering Time.Plants (Basel, Switzerland) · 2023Review
- The cytosolic iron-sulphur cluster assembly mechanism in grapevine is one target of a virulent Crinkler effector from Plasmopara viticola.Molecular plant pathology · 2022Article
- Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis.Plant physiology · 2022Article
- ROS1-mediated decrease in DNA methylation and increase in expression of defense genes and stress response genes in Arabidopsis thaliana due to abiotic stresses.BMC plant biology · 2022Article
- Fine mapping and characterization ofFrontiers in genetics · 2022Article
- Redox Components: Key Regulators of Epigenetic Modifications in Plants.International journal of molecular sciences · 2020Review
- Active DNA Demethylation in Plants.International journal of molecular sciences · 2019Review
- Histone acetylation recruits the SWR1 complex to regulate active DNA demethylation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Peroxisomal β-oxidation regulates histone acetylation and DNA methylation inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Canonical cytosolic iron-sulfur cluster assembly and non-canonical functions of DRE2 in Arabidopsis.PLoS genetics · 2019Article
- Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei.PLoS pathogens · 2018Article
- CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation.Nature communications · 2018Article
- Active DNA demethylation: mechanism and role in plant development.Plant cell reports · 2018Review
- Thiol Based Redox Signaling in Plant Nucleus.Frontiers in plant science · 2018Review
- Mitochondrial ABC Transporter ATM3 Is Essential for Cytosolic Iron-Sulfur Cluster Assembly.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
DNA demethylation mediated by the DNA glycosylase ROS1 helps determine genomic DNA methylation patterns and protects active genes from being silenced. However, little is known about the mechanism of regulation of ROS1 enzymatic activity. Using a forward genetic screen, we identified an anti-silencing (ASI) factor, ASI3, the dysfunction of which causes transgene promoter hyper-methylation and silencing. Map-based cloning identified ASI3 as MET18, a component of the cytosolic iron-sulfur cluster assembly (CIA) pathway. Mutation in MET18 leads to hyper-methylation at thousands of genomic loci, the majority of which overlap with hypermethylated loci identified in ros1 and ros1dml2dml3 mutants. Affinity purification followed by mass spectrometry indicated that ROS1 physically associates with MET18 and other CIA components. Yeast two-hybrid and split luciferase assays showed that ROS1 can directly interact with MET18 and another CIA component, AE7. Site-directed mutagenesis of ROS1 indicated that the conserved iron-sulfur motif is indispensable for ROS1 enzymatic activity. Our results suggest that ROS1-mediated active DNA demethylation requires MET18-dependent transfer of the iron-sulfur cluster, highlighting an important role of the CIA pathway in epigenetic regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.