ArticleAnalytical chemistry2015
Quantification of Borrelia burgdorferi Membrane Proteins in Human Serum: A New Concept for Detection of Bacterial Infection.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease.Journal of clinical microbiology · 2025Article
- Direct Detection of Lyme Borrelia: Recent Advancement and Use of Aptamer Technology.Biomedicines · 2023Review
- Lyme borreliosis diagnosis: state of the art of improvements and innovations.BMC microbiology · 2023Review
- A portable immunosensor provides sensitive and rapid detection of Borrelia burgdorferi antigen in spiked blood.Scientific reports · 2023Article
- Cross-Reactive Results in Serological Tests for Borreliosis in Patients with Active Viral Infections.Pathogens (Basel, Switzerland) · 2022Article
- Testing Raman spectroscopy as a diagnostic approach for Lyme disease patients.Frontiers in cellular and infection microbiology · 2022Article
- Immunoreactivity of Polish Lyme Disease Patient Sera to SpecificDiagnostics (Basel, Switzerland) · 2021Article
- Quantitative multiplexed strategies for human Lyme disease serological testing.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.Journal of proteome research · 2020Article
- Immunoserological Diagnosis of Human Borrelioses: Current Knowledge and Perspectives.Frontiers in cellular and infection microbiology · 2020Review
- Lyme Disease Frontiers: ReconcilingPathogens (Basel, Switzerland) · 2019Review
- Multi-platform Approach for Microbial Biomarker Identification UsingFrontiers in cellular and infection microbiology · 2019Article
- Recent strategies for the diagnosis of early Lyme disease.Science progress · 2018Review
- Five-Antigen Fluorescent Bead-Based Assay for Diagnosis of Lyme Disease.Clinical and vaccine immunology : CVI · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The Borrelia burgdorferi spirochete is the causative agent of Lyme disease, the most common tick-borne disease in the United States. The low abundance of bacterial proteins in human serum during infection imposes a challenge for early proteomic detection of Lyme disease. To address this challenge, we propose to detect membrane proteins released from bacteria due to disruption of their plasma membrane triggered by the innate immune system. These membrane proteins can be separated from the bulk of serum proteins by high-speed centrifugation causing substantial sample enrichment prior to targeted protein quantification using multiple reaction monitoring mass spectrometry. This new approach was first applied to detection of B. burgdorferi membrane proteins supplemented in human serum. Our results indicated that detection of B. burgdorferi membrane proteins, which are ≈10(7) lower in abundance than major serum proteins, is feasible. Therefore, quantitative analysis was also carried out for serum samples from three patients with acute Lyme disease. We were able to demonstrate the detection of ospA, the major B. burgdorferi lipoprotein at the level of 4.0 fmol of ospA/mg of serum protein. The results confirm the concept and suggest that the proposed approach can be expanded to detect other bacterial infections in humans, particularly where existing diagnostics are unreliable.
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