Evidence map›Paper›PMID 26491715›Full record

ArticleTheScientificWorldJournal2015

Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics.

Dyana Leal Veras, Ana Catarina de Souza Lopes, Grasielle Vaz da Silva, Gabriel Gazzoni Araújo Gonçalves, Catarina Fernandes de Freitas, Fernanda Cristina Gomes de Lima, Maria Amélia Vieira Maciel, Ana Paula Sampaio Feitosa, Luiz Carlos Alves, Fábio André Brayner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in TheScientificWorldJournal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Molecular Insights and Antibiotic Resistance ofFood science & nutrition · 2026
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dyana Leal VerasSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Enfermagem, Faculdade de Ciências Humanas de Olinda (FACHO), Rod PE-015, Jatobá, 53060-775 Olinda, PE, Brazil.
Ana Catarina de Souza LopesDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.
Grasielle Vaz da SilvaSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Rua Arnobio Marques 310, Santo Amaro, 50100-130 Recife, PE, Brazil.
Gabriel Gazzoni Araújo GonçalvesSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil.
Catarina Fernandes de FreitasDepartamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.
Fernanda Cristina Gomes de LimaDepartamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.
Maria Amélia Vieira MacielDepartamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.
Ana Paula Sampaio FeitosaSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.
Luiz Carlos AlvesSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Instituto de Ciências Biológicas, Universidade de Pernambuco (UPE), Rua Arnobio Marques 310, Santo Amaro, 50100-130 Recife, PE, Brazil.
Fábio André BraynerSetor de Microscopia Eletrônica, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil ; Departamento de Parasitologia, Centro de Pesquisas Aggeu Magalhães (CPqAM)-Fiocruz, Avenida Professor Moraes Rego, s/n, Caixa Postal 7472, Cidade Universitária, 50670-420 Recife, PE, Brazil ; Departamento de Medicina Tropical, Universidade Federal de Pernambuco (UFPE), Avenida Professor Moraes Rego, s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.

Indexed as

Genes, BacterialAnti-Bacterial Agentsbeta-Lactamasesbeta-LactamsHumansKlebsiella pneumoniaeMicrobial Sensitivity TestsMicrobiotaSequence Analysis, DNAAnti-Bacterial Agentsbeta-Lactamasesbeta-Lactams

Identifiers

PMID26491715
PMCPMC4600873

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.