ArticleMetabolomics : Official journal of the Metabolomic Society
A rapid method for profiling of volatile and semi-volatile phytohormones using methyl chloroformate derivatisation and GC-MS.
Article in Metabolomics : Official journal of the Metabolomic Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 40 citations in OpenAlex.
- Algal Growth Regulators: Releasing Plant Hormones for Sustainable Horticulture.Plants (Basel, Switzerland) · 2026Review
- Development and Validation of an LC-MS/MS Method for Quantifying Phytohormones Related to Tomato Shelf Life.Foods (Basel, Switzerland) · 2025Article
- Fertilization Strategies in Huanglongbing-InfectedPlants (Basel, Switzerland) · 2025Article
- Growth and Molecular Responses of Tomato to Prolonged and Short-Term Heat Exposure.International journal of molecular sciences · 2023Article
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- Unravelling Plant Responses to Stress-The Importance of Targeted and Untargeted Metabolomics.Metabolites · 2021Review
- Two Auxinic Herbicides AffectBiomolecules · 2021Article
- The Steroid Saponin Protodioscin ModulatesPlants (Basel, Switzerland) · 2021Article
- Coumarin Interferes with Polar Auxin Transport Altering Microtubule Cortical Array Organization inInternational journal of molecular sciences · 2021Article
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- Phytotoxicity, Morphological, and Metabolic Effects of the Sesquiterpenoid Nerolidol onPlants (Basel, Switzerland) · 2020Article
- Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review.Plant methods · 2020Review
- Phenotyping reproductive stage chilling and frost tolerance in wheat using targeted metabolome and lipidome profiling.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Mass spectrometry as a quantitative tool in plant metabolomics.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2016Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytohormones are central components of complex signalling networks in plants. The interplay between these metabolites, which include abscisic acid (ABA), auxin (IAA), ethylene, jasmonic acid (JA) and salicylic acid (SA), regulate plant growth and development and modulate responses to biotic and abiotic stress. Few methods of phytohormone profiling can adequately quantify a large range of plant hormones simultaneously and without the requirement for laborious or highly specialised extraction protocols. Here we describe the development and validation of a phytohormone profiling protocol, based on methyl-chloroformate derivatisation of the plant metabolites and analysis by gas chromatography/mass spectrometry (GC-MS). We describe the analysis of 11 metabolites, either plant phytohormones or intermediates of phytohormone metabolism; ABA, azelaic acid, IAA, JA and SA, and the phytohormone precursors 1-aminocyclopropane 1-carboxylic acid, benzoic acid, cinnamic acid, 13-epi-12-oxophytodienoic acid (13-epi-OPDA), linoleic acid and linolenic acid, and validate the isolation from foliar tissue of the model legume
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