ArticleBiology of reproduction2015
Disrupting Cyclin Dependent Kinase 1 in Spermatocytes Causes Late Meiotic Arrest and Infertility in Mice.
Article in Biology of reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Proteomic analysis of cisplatin-induced spermatogenesis defects in mice.Annals of medicine · 2026Article
- Testis-specific protein HSF5 is essential for proper chromatin organization and transcriptional reprogramming to drive pachynema progression.Zoological research · 2025Article
- Alkaloid fraction of Achyranthes aspera Linn triggers breast cancer apoptosis in mice (Open veterinary journal · 2025Article
- Actin-related protein ACTL7B ablation leads to OAT with multiple morphological abnormalities of the flagellum and male infertility in mice†.Biology of reproduction · 2023Article
- Deletion of NFIX results in defective progression through meiosis within the mouse testis†.Biology of reproduction · 2022Article
- The Spo13/Meikin pathway confines the onset of gamete differentiation to meiosis II in yeast.The EMBO journal · 2022Article
- Proteome Informatics in Tibetan Sheep (Frontiers in veterinary science · 2022Article
- Human MLH1/3 variants causing aneuploidy, pregnancy loss, and premature reproductive aging.Nature communications · 2021Article
- PRSS50 is a testis protease responsible for proper sperm tail formation and function.Development (Cambridge, England) · 2021Article
- Weighted Correlation Gene Network Analysis Reveals New Potential Mechanisms and Biomarkers in Non-obstructive Azoospermia.Frontiers in genetics · 2021Article
- The Role of CDKs and CDKIs in Murine Development.International journal of molecular sciences · 2020Review
- CDK2 kinase activity is a regulator of male germ cell fate.Development (Cambridge, England) · 2019Article
- Diverse roles for CDK-associated activity during spermatogenesis.FEBS letters · 2019Review
- Article
- Functions of cyclins and CDKs in mammalian gametogenesis†.Biology of reproduction · 2019Review
- FTO Knockout Causes Chromosome Instability and G2/M Arrest in Mouse GC-1 Cells.Frontiers in genetics · 2018Article
- Requirement for CCNB1 in mouse spermatogenesis.Cell death & disease · 2017Article
- Cross-talk between sumoylation and phosphorylation in mouse spermatocytes.Biochemical and biophysical research communications · 2017Article
- Testicular abnormalities in mice with Y chromosome deficiencies.Biology of reproduction · 2017Article
- Aminopurvalanol A, a Potent, Selective, and Cell Permeable Inhibitor of Cyclins/Cdk Complexes, Causes the Reduction ofFrontiers in physiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
While cyclin dependent kinase 1 (CDK1) has a critical role in controlling resumption of meiosis in oocytes, its role has not been investigated directly in spermatocytes. Unique aspects of male meiosis led us to hypothesize that its role is different in male meiosis than in female meiosis. We generated a conditional knockout (cKO) of the Cdk1 gene in mouse spermatocytes to test this hypothesis. We found that CDK1-null spermatocytes undergo synapsis, chiasmata formation, and desynapsis as is seen in oocytes. Additionally, CDK1-null spermatocytes relocalize SYCP3 to centromeric foci, express H3pSer10, and initiate chromosome condensation. However, CDK1-null spermatocytes fail to form condensed bivalent chromosomes in prophase of meiosis I and instead are arrested at prometaphase. Thus, CDK1 has an essential role in male meiosis that is consistent with what is known about the role of CDK1 in female meiosis, where it is required for formation of condensed bivalent metaphase chromosomes and progression to the first meiotic division. We found that cKO spermatocytes formed fully condensed bivalent chromosomes in the presence of okadaic acid, suggesting that cKO chromosomes are competent to condense, although they do not do so in vivo. Additionally, arrested cKO spermatocytes exhibited irregular cell shape, irregular large nuclei, and large distinctive nucleoli. These cells persist in the seminiferous epithelium through the next seminiferous epithelial cycle with a lack of stage XII checkpoint-associated cell death. This indicates that CDK1 is required upstream of a checkpoint-associated cell death as well as meiotic metaphase progression in mouse spermatocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.