Evidence map›Paper›PMID 26490839›Full record

ArticleBiology of reproduction2015

Progesterone Accelerates the Completion of Sperm Capacitation and Activates CatSper Channel in Spermatozoa from the Rhesus Macaque.

Shiho Sumigama, Steven Mansell, Melissa Miller, Polina V Lishko, Gary N Cherr, Stuart A Meyers, Theodore Tollner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shiho SumigamaDepartment of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California.
Steven MansellDepartment of Molecular and Cell Biology, University of California, Berkeley, California.
Melissa MillerDepartment of Molecular and Cell Biology, University of California, Berkeley, California.
Polina V LishkoDepartment of Molecular and Cell Biology, University of California, Berkeley, California.
Gary N CherrBodega Marine Laboratory, Departments of Environmental Toxicology and Nutrition, University of California, Davis, California.
Stuart A MeyersDepartment of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, California smeyers@ucdavis.edu.
Theodore TollnerDepartment of Obstetrics and Gynecology, School of Medicine, University of California, Davis, California Center for Health and the Environment, University of California, Davis, California.

Funding

Response of Rhesus Sperm to Freezing: Recovery from Osmotic and Oxidative StressR01RR016581 · NCRR · UNIVERSITY OF CALIFORNIA DAVIS · PI MEYERS, STUART A · 2002 to 2010
$2.6M
NCRR NIH HHS R01 RR016581NCRR NIH HHS R01RR016581
6 · The paper itself

Abstract

During transit through the female reproductive tract, mammalian spermatozoa are exposed to increasing concentrations of progesterone (P4) released by the cumulus oophorus. P4 triggers massive calcium influx into human sperm through activation of the sperm-specific calcium channel CatSper. These properties of human spermatozoa are thought to be unique since CatSper is not progesterone sensitive in rodent sperm. Here, by performing patch clamp recording from spermatozoa from rhesus macaque for the first time, we report that they express P4-sensitive CatSper channel identically to human sperm and react to P4 by inducing responsiveness to zona pellucida, unlike human sperm, which respond directly to P4. We have also determined the physiologic levels of P4 capable of inducing capacitation-associated changes in macaque sperm. Progesterone (1 μM) induced up to a 3-fold increase in the percentage of sperm undergoing the zona pellucida-induced acrosome reaction with the lowest threshold as low as 10 nM of P4. Submicromolar levels of P4 induced a dose-dependent increase in curvilinear velocity and lateral head displacement, while sperm protein tyrosine phosphorylation was not altered. Macaque spermatozoa exposed to 10 μM of P4 developed fully hyperactivated motility. Similar to human sperm, on approaching cumulus mass and binding to zona pellucida, macaque spermatozoa display hyperactivation and undergo an acrosome reaction that coincides with the rise in the sperm intracellular calcium. Taken together, these data indicate that P4 accelerates the completion of capacitation and provides evidence of spermatozoa "priming" as they move into a gradient of progesterone in search for the oocyte.

Indexed as

AnimalsCalciumCalcium ChannelsDose-Response Relationship, DrugMacaca mulattaMalePhosphorylationProgesteroneReceptors, AndrogenSpermatozoaSperm CapacitationSperm MotilityZona PellucidaCalciumCalcium ChannelsProgesteroneReceptors, AndrogenCatSper ion channelhyperactivationprogesteronesperm capacitationzona-induced acrosome reaction

Identifiers

PMID26490839
PMCPMC4712691

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.