ArticleScientific reports2015
Engineering a pyridoxal 5'-phosphate supply for cadaverine production by using Escherichia coli whole-cell biocatalysis.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 78 citations in OpenAlex.
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- Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli.BMC biotechnology · 2023Article
- Efficient and scalable synthesis of 1,5-diamino-2-hydroxy-pentane from L-lysine via cascade catalysis using engineered Escherichia coli.Microbial cell factories · 2022Article
- Utilization of a Wheat Sidestream for 5-Aminovalerate Production inFrontiers in bioengineering and biotechnology · 2021Article
- Bacterial synthesis of C3-C5 diols via extending amino acid catabolism.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization.Frontiers in bioengineering and biotechnology · 2020Article
- Identification of new glutamate decarboxylases fromJournal of biological engineering · 2019Article
- Methanol fermentation increases the production of NAD(P)H-dependent chemicals in synthetic methylotrophicBiotechnology for biofuels · 2019Article
- Expanding lysine industry: industrial biomanufacturing of lysine and its derivatives.Journal of industrial microbiology & biotechnology · 2018Review
- A Novel Process for Cadaverine Bio-Production Using a Consortium of Two EngineeredFrontiers in microbiology · 2018Article
- Whole-cell biocatalysts by design.Microbial cell factories · 2017Review
- Improvement of whole-cell transamination with Saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation.Microbial cell factories · 2017Article
- Pyridoxal phosphate synthases PdxS/PdxT are required for Actinobacillus pleuropneumoniae viability, stress tolerance and virulence.PloS one · 2017Article
- Lysine Decarboxylase with an Enhanced Affinity for Pyridoxal 5-Phosphate by Disulfide Bond-Mediated Spatial Reconstitution.PloS one · 2017Article
- Crystal Structure and Pyridoxal 5-Phosphate Binding Property of Lysine Decarboxylase from Selenomonas ruminantium.PloS one · 2016Article
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7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the routes of de novo pyridoxal 5'-phosphate (PLP) biosynthesis have been well described, studies of the engineering of an intracellular PLP supply are limited, and the effects of cellular PLP levels on PLP-dependent enzyme-based whole-cell biocatalyst activity have not been described. To investigate the effects of PLP cofactor availability on whole-cell biocatalysis, the ribose 5-phosphate (R5P)-dependent pathway genes pdxS and pdxT of Bacillus subtilis were introduced into the lysine decarboxylase (CadA)-overexpressing Escherichia coli strain BL-CadA. This strain was then used as a whole-cell biocatalyst for cadaverine production from L-lysine. Co-expression strategies were evaluated, and the culture medium was optimised to improve the biocatalyst performance. As a result, the intracellular PLP concentration reached 1144 nmol/gDCW, and a specific cadaverine productivity of 25 g/gDCW/h was achieved; these values were 2.4-fold and 2.9-fold higher than those of unmodified BL-CadA, respectively. Additionally, the resulting strain AST3 showed a cadaverine titre (p = 0.143, α = 0.05) similar to that of the BL-CadA strain with the addition of 0.1 mM PLP. These approaches for improving intracellular PLP levels to enhance whole-cell lysine bioconversion activity show great promise for the engineering of a PLP cofactor to optimise whole-cell biocatalysis.
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