ArticleBMC genomics2015
Plasmodium parasites mount an arrest response to dihydroartemisinin, as revealed by whole transcriptome shotgun sequencing (RNA-seq) and microarray study.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- Parasite clearance in patients with Plasmodium vivax monoinfection treated with artesunate in Cambodia: an observational secondary analysis of trial data.The Lancet. Microbe · 2026Observational
- Understanding the Antiplasmodial Action of Resistance-Refractory Xanthoquinodin A1.ACS infectious diseases · 2024Article
- Article
- Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella.Parasitology research · 2022Article
- Artemisinin resistance in the malaria parasite, Plasmodium falciparum, originates from its initial transcriptional response.Communications biology · 2022Article
- Article
- Molecular characterization and functional analysis of Eimeria tenella citrate synthase.Parasitology research · 2021Article
- Artemisinin-resistant K13 mutations rewire Plasmodium falciparum's intra-erythrocytic metabolic program to enhance survival.Nature communications · 2021Article
- Describing the current status of Plasmodium falciparum population structure and drug resistance within mainland Tanzania using molecular inversion probes.Molecular ecology · 2021Article
- Molecular Mechanisms of Drug Resistance inAnnual review of microbiology · 2020Review
- A Biosynthetic Platform for Antimalarial Drug Discovery.Antimicrobial agents and chemotherapy · 2020Article
- Insights into the intracellular localization, protein associations and artemisinin resistance properties of Plasmodium falciparum K13.PLoS pathogens · 2020Article
- From Genes to Transcripts, a Tightly Regulated Journey inFrontiers in cellular and infection microbiology · 2020Review
- A fast and cost-effective microsampling protocol incorporating reduced animal usage for time-series transcriptomics in rodent malaria parasites.Malaria journal · 2019Article
- Validation ofPeerJ · 2019Article
- Molecular and cellular characterization of apoptosis in flat oyster a key mechanisms at the heart of host-parasite interactions.Scientific reports · 2018Article
- Inhibiting the Plasmodium eIF2α Kinase PK4 Prevents Artemisinin-Induced Latency.Cell host & microbe · 2017Article
- Antimalarial Drug Resistance: A Threat to Malaria Elimination.Cold Spring Harbor perspectives in medicine · 2017Review
- Knockout of the peroxiredoxin 5 homologue PFAOP does not affect the artemisinin susceptibility of Plasmodium falciparum.Scientific reports · 2017Article
- Correlation between Cyclin Dependent Kinases and Artemisinin-Induced Dormancy in Plasmodium falciparum In Vitro.PloS one · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundControl of malaria is threatened by emerging parasite resistance to artemisinin and derivative drug (ART) therapies. The molecular detail of how Plasmodium malaria parasites respond to ART and how this could contribute to resistance are not well understood. To address this question, we performed a transcriptomic study of dihydroartemisinin (DHA) response in P. falciparum K1 strain and in P. berghei ANKA strain using microarray and RNA-seq technology.
resultsMicroarray data from DHA-treated P. falciparum trophozoite stage parasites revealed a response pattern that is overall less trophozoite-like and more like the other stages of asexual development. A meta-analysis of these data with previously published data from other ART treatments revealed a set of common differentially expressed genes. Notably, ribosomal protein genes are down-regulated in response to ART. A similar pattern of trophozoite transcriptomic change was observed from RNA-seq data. RNA-seq data from DHA-treated P. falciparum rings reveal a more muted response, although there is considerable overlap of differentially expressed genes with DHA-treated trophozoites. No genes are differentially expressed in DHA-treated P. falciparum schizonts. The transcriptional response of P. berghei to DHA treatment in vivo in infected mice is similar to the P. falciparum in vitro culture ring and trophozoite responses, in which ribosomal protein genes are notably down-regulated.
conclusionsRing and trophozoite stage Plasmodium respond to ART by arresting metabolic processes such as protein synthesis and glycolysis. This response can be protective in rings, as shown by the phenomenon of dormancy. In contrast, this response is not as protective in trophozoites owing to their commitment to a highly active and vulnerable metabolic state. The lower metabolic demands of schizonts could explain why they are less sensitive and unresponsive to ART. The ART response pattern is revealed clearly from RNA-seq data, suggesting that this technology is of great utility for studying drug response in Plasmodium.
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