ArticlePloS one2015
In Vitro Generation of Functional Liver Organoid-Like Structures Using Adult Human Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 109 citations in OpenAlex.
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- Microfluidic Liver-on-a-Chip for Preclinical Drug Discovery.Pharmaceutics · 2023Review
- Review
- Vascularized Tissue Organoids.Bioengineering (Basel, Switzerland) · 2023Review
- Organoids revealed: morphological analysis of the profound next generation in-vitro model with artificial intelligence.Bio-design and manufacturing · 2023Review
- Recent advances in organoid engineering: A comprehensive review.Applied materials today · 2022Article
- Inventing Engineered Organoids for end-stage liver failure patients.Journal of molecular histology · 2022Review
- MatriGridBioengineering (Basel, Switzerland) · 2022Review
- Establishment of large canine hepatocyte spheroids by mixing vascular endothelial cells and canine adipose-derived mesenchymal stem cells.Regenerative therapy · 2022Article
- Coordination of iron homeostasis by bone morphogenetic proteins: Current understanding and unanswered questions.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications.Frontiers in bioengineering and biotechnology · 2022Review
- Physiologically relevant microsystems to study viral infection in the human liver.Frontiers in microbiology · 2022Review
- Human Brain-Based Models Provide a Powerful Tool for the Advancement of Parkinson's Disease Research and Therapeutic Development.Frontiers in neuroscience · 2022Review
- Retinal organoids on-a-chip: a micro-millifluidic bioreactor for long-term organoid maintenance.Lab on a chip · 2021Article
- Generation of Scalable Hepatic Micro-Tissues as a Platform for Toxicological Studies.Tissue engineering and regenerative medicine · 2020Article
- Synthetic alternatives to Matrigel.Nature reviews. Materials · 2020Article
- Mechanical Stimulation: A Crucial Element of Organ-on-Chip Models.Frontiers in bioengineering and biotechnology · 2020Review
- Review
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we used differentiated adult human upcyte® cells for the in vitro generation of liver organoids. Upcyte® cells are genetically engineered cell strains derived from primary human cells by lenti-viral transduction of genes or gene combinations inducing transient proliferation capacity (upcyte® process). Proliferating upcyte® cells undergo a finite number of cell divisions, i.e., 20 to 40 population doublings, but upon withdrawal of proliferation stimulating factors, they regain most of the cell specific characteristics of primary cells. When a defined mixture of differentiated human upcyte® cells (hepatocytes, liver sinusoidal endothelial cells (LSECs) and mesenchymal stem cells (MSCs)) was cultured in vitro on a thick layer of Matrigel™, they self-organized to form liver organoid-like structures within 24 hours. When further cultured for 10 days in a bioreactor, these liver organoids show typical functional characteristics of liver parenchyma including activity of cytochromes P450, CYP3A4, CYP2B6 and CYP2C9 as well as mRNA expression of several marker genes and other enzymes. In summary, we hereby describe that 3D functional hepatic structures composed of primary human cell strains can be generated in vitro. They can be cultured for a prolonged period of time and are potentially useful ex vivo models to study liver functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.