ArticleJournal of biophotonics2016
High speed sCMOS-based oblique plane microscopy applied to the study of calcium dynamics in cardiac myocytes.
Article in Journal of biophotonics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- High content 3D imaging by dual-view oblique plane microscopy.PNAS nexus · 2025Article
- High-Throughput, Low Background, and Wide-Field Microscopy by Flat-Field Photobleaching Imprinting Microscopy.Chemical & biomedical imaging · 2023Article
- Blazed oblique plane microscopy reveals scale-invariant inference of brain-wide population activity.Nature communications · 2023Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Remote-refocusing light-sheet fluorescence microscopy enables 3D imaging of electromechanical coupling of hiPSC-derived and adult cardiomyocytes in co-culture.Scientific reports · 2023Article
- High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes.Frontiers in physiology · 2023Article
- SOLEIL: single-objective lens inclined light sheet localization microscopy.Biomedical optics express · 2022Article
- Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lenses.Quantitative imaging in medicine and surgery · 2021Article
- Contemporary Whole Slide Imaging Devices and Their Applications within the Modern Pathology Department: A Selected Hardware Review.Journal of pathology informatics · 2021Review
- Dual-view oblique plane microscopy (dOPM).Biomedical optics express · 2020Article
- Tilt (in)variant lateral scan in oblique plane microscopy: a geometrical optics approach.Biomedical optics express · 2020Article
- Fast objective coupled planar illumination microscopy.Nature communications · 2019Article
- Tilt-invariant scanned oblique plane illumination microscopy for large-scale volumetric imaging.Optics letters · 2019Article
- Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging.Optics express · 2018Article
- Volumetric fluorescence retinal imagingBiomedical optics express · 2018Article
- Hierarchical statistical techniques are necessary to draw reliable conclusions from analysis of isolated cardiomyocyte studies.Cardiovascular research · 2017Article
- Reflective imaging improves spatiotemporal resolution and collection efficiency in light sheet microscopy.Nature communications · 2017Article
- Lossless Three-Dimensional Parallelization in Digitally Scanned Light-Sheet Fluorescence Microscopy.Scientific reports · 2017Article
- Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan.Scientific reports · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Oblique plane microscopy (OPM) is a form of light sheet microscopy that uses a single high numerical aperture microscope objective for both fluorescence excitation and collection. In this paper, measurements of the relative collection efficiency of OPM are presented. An OPM system incorporating two sCMOS cameras is then introduced that enables single isolated cardiac myocytes to be studied continuously for 22 seconds in two dimensions at 667 frames per second with 960 × 200 pixels and for 30 seconds with 960 × 200 × 20 voxels at 25 volumes per second. In both cases OPM is able to record in two spectral channels, enabling intracellular calcium to be studied via the probe Fluo-4 AM simultaneously with the sarcolemma and transverse tubule network via the membrane dye Cellmask Orange. The OPM system was then applied to determine the spatial origin of spontaneous calcium waves for the first time and to measure the cell transverse tubule structure at their point of origin. Further results are presented to demonstrate that the OPM system can also be used to study calcium spark parameters depending on their relationship to the transverse tubule structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.