ArticleThe Journal of biological chemistry2015
Normal Fertility Requires the Expression of Carbonic Anhydrases II and IV in Sperm.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Catsper channel and its roles in male fertility: a systematic review.Reproductive biology and endocrinology : RB&E · 2017Pooled it
- Topiramate Impairs Spermatogenesis, Sperm Parameters, and Epididymal Duct Contractility in Rats.ACS pharmacology & translational science · 2026Article
- The systems that transport ions and their impact on sperm function and male fertility.Function (Oxford, England) · 2026Review
- Coordinated and compartmentalized functions of CAII and CAIV establish a dual pH-regulatory mechanism essential for sperm maturation and capacitation.bioRxiv : the preprint server for biology · 2025Article
- Beneficial Effects of Resveratrol on Testicular Functions: Focus on Its Antioxidant Properties.Cells · 2025Review
- Comparative transcriptome analysis of bull X- and Y-spermatozoa.Scientific reports · 2025Article
- Review
- Control of intracellular pH and bicarbonate by CONature communications · 2023Article
- N-thiocarboxyanhydrides, amino acid-derived enzyme-activated HBMC veterinary research · 2023Article
- Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens.Frontiers in physiology · 2023Article
- The Role of Long Noncoding RNAs on Male Infertility: A Systematic Review and In Silico Analysis.Biology · 2022Review
- A Review on the Role of Bicarbonate and Proton Transporters during Sperm Capacitation in Mammals.International journal of molecular sciences · 2022Review
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- A Minimal Model Shows that a Positive Feedback Loop Between sNHE and SLO3 can Control Mouse Sperm Capacitation.Frontiers in cell and developmental biology · 2022Article
- Dissecting the signaling pathways involved in the function of sperm flagellum.Current opinion in cell biology · 2020Review
- The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.EMBO reports · 2019Article
- The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.Cell reports · 2018Article
- CFTR/ENaC-dependent regulation of membrane potential during human sperm capacitation is initiated by bicarbonate uptake through NBC.The Journal of biological chemistry · 2018Article
- Insights into pH regulatory mechanisms in mediating spermatozoa functions.Veterinary world · 2018Review
- A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection.Tropical animal health and production · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HCO3 (-) is a key factor in the regulation of sperm motility. High concentrations of HCO3 (-) in the female genital tract induce an increase in sperm beat frequency, which speeds progress of the sperm through the female reproductive tract. Carbonic anhydrases (CA), which catalyze the reversible hydration of CO2 to HCO3 (-), represent potential candidates in the regulation of the HCO3 (-) homeostasis in sperm and the composition of the male and female genital tract fluids. We show that two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty. Expression analyses reveal an up-regulation of CAII and CAIV in the different epididymal sections of the knockout lines. In sperm, we find that CAII is located in the principal piece, whereas CAIV is present in the plasma membrane of the entire sperm tail. CAII and CAIV single knockout animals display an imbalanced HCO3 (-) homeostasis, resulting in substantially reduced sperm motility, swimming speed, and HCO3 (-)-enhanced beat frequency. The CA activity remaining in the sperm of CAII- and CAIV-null mutants is 35% and 68% of that found in WT mice. Sperm of the double knockout mutant mice show responses to stimulus by HCO3 (-) or CO2 that were delayed in onset and reduced in magnitude. In comparison with sperm from CAII and CAIV double knockout animals, pharmacological loss of CAIV in sperm from CAII knockout animals, show an even lower response to HCO3 (-). These results suggest that CAII and CAIV are required for optimal fertilization.
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