ArticleProtein engineering, design & selection : PEDS2015
Reconstitution of the receptor-binding motif of the SARS coronavirus.
Article in Protein engineering, design & selection : PEDS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- SARS-CoV-2 spike protein reduces burst activities in neurons measured by micro-electrode arrays.Annals of medicine and surgery (2012) · 2023Article
- Secretory Phospholipase A2 and Interleukin-6 Levels as Predictive Markers of the Severity and Outcome of Patients with COVID-19 Infections.International journal of molecular sciences · 2023Article
- Baculovirus displaying SARS-CoV-2 spike RBD promotes neutralizing antibody production in a mouse model.Journal, genetic engineering & biotechnology · 2023Article
- Functional reconstitution of the MERS CoV receptor binding motif.Molecular immunology · 2022Article
- Phage Display Technique as a Tool for Diagnosis and Antibody Selection for Coronaviruses.Current microbiology · 2021Review
- Genetic Risk Factors for the Development of COVID-19 Coronavirus Infection.Russian journal of genetics · 2021Article
- Smart polymeric eye gear: A possible preventive measure against ocular transmission of COVID-19.Medical hypotheses · 2020Article
- [Comparative analysis of structural characteristics and epitopes in S proteins between SARS-CoV-2 and SARS-CoV].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2020Article
- The 2019 coronavirus: Learning curves, lessons, and the weakest link.International journal of clinical practice · 2020Article
- Oral disease-modifying antirheumatic drugs and immunosuppressants with antiviral potential, including SARS-CoV-2 infection: a review.Therapeutic advances in musculoskeletal disease · 2020Article
- B-cell restriction - an alternative piece to the puzzle.Human vaccines & immunotherapeutics · 2019Article
- Targeting endosomal acidification by chloroquine analogs as a promising strategy for the treatment of emerging viral diseases.Pharmacology research & perspectives · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The severe acute respiratory syndrome (SARS) coronavirus (CoV) identified in 2003 has infected ∼8000 people worldwide, killing nearly 10% of them. The infection of target cells by the SARS CoV is mediated through the interaction of the viral Spike (S) protein (1255 amino acids) and its cellular receptor, angiotensin-converting enzyme 2 (ACE2). The SARS CoV receptor-binding domain (amino acids N318-T509 of S protein) harbors an extended excursion along its periphery that contacts ACE2 and is designated the receptor-binding motif (RBM, amino acids S432-T486). In addition, the RBM is a major antigenic determinant, able to elicit production of neutralizing antibodies. Hence, the role of the RBM is a bi-functional bioactive surface that can be demonstrated by antibodies such as the neutralizing human anti-SARS monoclonal antibody (mAb) 80R which targets the RBM and competes with the ACE2 receptor for binding. Here, we employ phage-display peptide-libraries to reconstitute a functional RBM. This is achieved by generating a vast collection of candidate RBM peptides that present a diversity of conformations. Screening such 'Conformer Libraries' with corresponding ligands has produced short RBM constructs (ca. 40 amino acids) that can bind both the ACE2 receptor and the neutralizing mAb 80R.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.