ArticlePlant physiology2015
Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35citing papers in PubMed
4.5field-weighted citation impact, top 5% of its field
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Who cites it
35 citing papers in PubMed, 138 citations in OpenAlex.
- Cross-species dissection of saline-related genes by genetically deciphering a euryhaline microalga Chlorella sp.Nature communications · 2026Article
- Management of growth and astaxanthin accumulation in the green microalgae Haematococcus pluvialis: effect of photosynthesis-related nutrients and light stress.Antonie van Leeuwenhoek · 2025Article
- Chromosome-level genome assembly for an edible protein microalgae Auxenochlorella pyrenoidosa.Scientific data · 2025Article
- Phylogenetic analysis of pathogenic algae reveals lineage-dependent patterns of phagocytosis.mBio · 2025Article
- Low-dose ionizing radiation generates a hormetic response to modify lipid metabolism in Chlorella sorokiniana.Communications biology · 2024Article
- Near telomere-to-telomere genome assemblies of two Chlorella species unveil the composition and evolution of centromeres in green algae.BMC genomics · 2024Article
- Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae.Microbial cell factories · 2023Review
- Strategies of NaCl Tolerance in Saline-Alkali-Tolerant Green MicroalgaPlants (Basel, Switzerland) · 2023Article
- Target of Rapamycin Regulates Photosynthesis and Cell Growth inInternational journal of molecular sciences · 2022Article
- Effective fucoxanthin production in the flagellate algaFrontiers in bioengineering and biotechnology · 2022Article
- Article
- The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Article
- Current perspectives on integrated approaches to enhance lipid accumulation in microalgae.3 Biotech · 2021Review
- Establishment of a Genome Editing Tool Using CRISPR-Cas9 inInternational journal of molecular sciences · 2021Article
- Metabolic Engineering for Carotenoid Production Using Eukaryotic Microalgae and Prokaryotic Cyanobacteria.Advances in experimental medicine and biology · 2021Article
- Trophic Transition Enhanced Biomass and Lipid Production of the Unicellular Green AlgaFrontiers in bioengineering and biotechnology · 2021Article
- The Structural Difference of IsobaricFrontiers in plant science · 2021Article
- Overexpression of the Transcription FactorFrontiers in bioengineering and biotechnology · 2021Article
- De novo transcriptome analysis of Chlorella sorokiniana: effect of glucose assimilation, and moderate light intensity.Scientific reports · 2020Article
- Comprehensive Utilization of Marine Microalgae for Enhanced Co-Production of Multiple Compounds.Marine drugs · 2020Review
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Authors and funding
24 authors at 3 institutions in 1 country.
Jianhua FanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Kang NingState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Xiaowei ZengState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Yuanchan LuoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Dongmei WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Jianqiang HuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Jing LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Hui XuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Jianke HuangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Minxi WanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Weiliang WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Daojing ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Guomin ShenState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Conglin RunState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Junjie LiaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Lei FangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Shi HuangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Xiaoyan JingState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Xiaoquan SuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Anhui WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Lili BaiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Zanmin HuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.).
Jian XuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.) ygli@ecust.edu.cn xujian@qibebt.ac.cn.
Yuanguang LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China (J.F., Y.Lu., H.X., J.Hua., M.W., W.W., D.Z., G.S., C.R., J.Lia., L.F., Y.Li.);Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China (K.N., X.Z., D.W., J.Hu., J.Li., S.H., X.J., X.S., A.W., J.X.); andInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (L.B., Z.H.) ygli@ecust.edu.cn xujian@qibebt.ac.cn.
East China University of Science and Technology · CNChinese Academy of Sciences · CNQingdao Institute of Bioenergy and Bioprocess Technology · CN
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to rapidly switch the intracellular energy storage form from starch to lipids is an advantageous trait for microalgae feedstock. To probe this mechanism, we sequenced the 56.8-Mbp genome of Chlorella pyrenoidosa FACHB-9, an industrial production strain for protein, starch, and lipids. The genome exhibits positive selection and gene family expansion in lipid and carbohydrate metabolism and genes related to cell cycle and stress response. Moreover, 10 lipid metabolism genes might be originated from bacteria via horizontal gene transfer. Transcriptomic dynamics tracked via messenger RNA sequencing over six time points during metabolic switch from starch-rich heterotrophy to lipid-rich photoautotrophy revealed that under heterotrophy, genes most strongly expressed were from the tricarboxylic acid cycle, respiratory chain, oxidative phosphorylation, gluconeogenesis, glyoxylate cycle, and amino acid metabolisms, whereas those most down-regulated were from fatty acid and oxidative pentose phosphate metabolism. The shift from heterotrophy into photoautotrophy highlights up-regulation of genes from carbon fixation, photosynthesis, fatty acid biosynthesis, the oxidative pentose phosphate pathway, and starch catabolism, which resulted in a marked redirection of metabolism, where the primary carbon source of glycine is no longer supplied to cell building blocks by the tricarboxylic acid cycle and gluconeogenesis, whereas carbon skeletons from photosynthesis and starch degradation may be directly channeled into fatty acid and protein biosynthesis. By establishing the first genetic transformation in industrial oleaginous C. pyrenoidosa, we further showed that overexpression of an NAD(H) kinase from Arabidopsis (Arabidopsis thaliana) increased cellular lipid content by 110.4%, yet without reducing growth rate. These findings provide a foundation for exploiting the metabolic switch in microalgae for improved photosynthetic production of food and fuels.
Indexed as
GenomicsLipid MetabolismBase SequenceCarbohydrate MetabolismCarbonChlorellaCitric Acid CycleElectron TransportFatty AcidsHeterotrophic ProcessesMolecular Sequence DataOxidative PhosphorylationPhotosynthesisSequence Analysis, DNAStarchCarbonFatty AcidsStarch
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