ArticleScientific reports2015
Selection of suitable reference genes for normalization of quantitative RT-PCR in peripheral blood samples of bottlenose dolphins (Tursiops truncatus).
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 34 citations in OpenAlex.
- Portable Molecular Diagnostics for Cetacean Morbillivirus: Development of a Reverse Transcription Insulated Isothermal PCR (RT-iiPCR) for Global Surveillance.Transboundary and emerging diseases · 2026Article
- Alzheimer's disease signatures in the brain transcriptome of Estuarine Dolphins.Communications biology · 2025Article
- TDP-43 and Alzheimer's Disease Pathology in the Brain of a Harbor Porpoise Exposed to the Cyanobacterial Toxin BMAA.Toxins · 2024Article
- Identification of Optimal Reference Genes for qRT-PCR Normalization for Physical Activity Intervention and Omega-3 Fatty Acids Supplementation in Humans.International journal of molecular sciences · 2023Article
- A Field-Deployable Insulated Isothermal PCR (iiPCR) for the Global Surveillance ofAnimals : an open access journal from MDPI · 2022Article
- Using transcriptomics to predict and visualize disease status in bighorn sheep (Conservation physiology · 2022Article
- Selection of a reference gene for studies on lipid-related aquatic adaptations of toothed whales (Ecology and evolution · 2021Article
- Gene Expression Profiles in Two Razor Clam Populations: Discerning Drivers of Population Status.Life (Basel, Switzerland) · 2021Article
- BMAA, Methylmercury, and Mechanisms of Neurodegeneration in Dolphins: A Natural Model of Toxin Exposure.Toxins · 2021Article
- Reliable reference genes for the quantification of mRNA in human T-cells and PBMCs stimulated with live influenza virus.BMC immunology · 2020Article
- Monitoring nearshore ecosystem health using Pacific razor clams (Siliqua patula) as an indicator species.PeerJ · 2020Article
- T Helper Cell Subsets and Their Functions in Common Bottlenose Dolphins (Frontiers in immunology · 2019Article
- Evaluation of Reference Genes for Normalization of RT-qPCR Gene Expression Data for Trichoplusia ni Cells During Antheraea pernyi (Lepidoptera: Saturniidae) Multicapsid Nucleopolyhedrovirus (AnpeNPV) Infection.Journal of insect science (Online) · 2019Article
- Sound exposure-induced cytokine gene transcript profile changes in captive bottlenose dolphin (Tursiops truncatus) blood identified by a probe-based qRT-PCR.The Journal of veterinary medical science · 2018Article
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- Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction.Scientific reports · 2016Article
- Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus.Scientific reports · 2016Article
- Selection of reference genes for RT-qPCR studies in blood of beluga whales (Delphinapterus leucas).PeerJ · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative RT-PCR is often used as a research tool directed at gene transcription. Selection of optimal housekeeping genes (HKGs) as reference genes is critical to establishing sensitive and reproducible qRT-PCR-based assays. The current study was designed to identify the appropriate reference genes in blood leukocytes of bottlenose dolphins (Tursiops truncatus) for gene transcription research. Seventy-five blood samples collected from 7 bottlenose dolphins were used to analyze 15 candidate HKGs (ACTB, B2M, GAPDH, HPRT1, LDHB, PGK1, RPL4, RPL8, RPL18, RPS9, RPS18, TFRC, YWHAZ, LDHA, SDHA). HKG stability in qRT-PCR was determined using geNorm, NormFinder, BestKeeper and comparative delta Ct algorithms. Utilization of RefFinder, which combined all 4 algorithms, suggested that PGK1, HPRT1 and RPL4 were the most stable HKGs in bottlenose dolphin blood. Gene transcription perturbations in blood can serve as an indication of health status in cetaceans as it occurs prior to alterations in hematology and chemistry. This study identified HKGs that could be used in gene transcript studies, which may contribute to further mRNA relative quantification research in the peripheral blood leukocytes in captive cetaceans.
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