ArticleDevelopmental biology2016
Clarification of mammalian cloacal morphogenesis using high-resolution episcopic microscopy.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- The cloacal outgrowth orchestrates co-development of the bladder and umbilical arteries.Development (Cambridge, England) · 2026Article
- Article
- Tgfbr1 controls developmental plasticity between the hindlimb and external genitalia by remodeling their regulatory landscape.Nature communications · 2024Article
- Effects of the environment on the evolution of the vertebrate urinary tract.Nature reviews. Urology · 2023Review
- Quantitative Image Processing for Three-Dimensional Episcopic Images of Biological Structures: Current State and Future Directions.Biomedicines · 2023Review
- Pathogenesis of Anorectal Malformations in Retinoic Acid Receptor Knockout Mice Studied by HREM.Biomedicines · 2021Article
- Duplex kidney formation: developmental mechanisms and genetic predisposition.F1000Research · 2020Review
- The development of the cloaca in the human embryo.Journal of anatomy · 2018Article
- Regulation of masculinization: androgen signalling for external genitalia development.Nature reviews. Urology · 2018Review
- Prostate organogenesis: tissue induction, hormonal regulation and cell type specification.Development (Cambridge, England) · 2017Review
- Stage- and subunit-specific functions of polycomb repressive complex 2 in bladder urothelial formation and regeneration.Development (Cambridge, England) · 2017Article
- Temporally Distinct Six2-Positive Second Heart Field Progenitors Regulate Mammalian Heart Development and Disease.Cell reports · 2017Article
- Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Temporal, spatial, and genetic regulation of external genitalia development.Differentiation; research in biological diversityReview
- Persistent Cloaca and Cloacal Variants in Males: Qualitative Review of a Neglected Anomaly.Journal of Indian Association of Pediatric SurgeonsArticle
- The embryology of persistent cloaca and urogenital sinus malformations.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The developmental process through which the cloaca transforms from one hollow structure to two separated urinary and digestive outlets remains controversial and speculative. Here, we use high-resolution episcopic microscopy to examine a comprehensive series of normal and mutant mouse cloaca in which the detailed 3-dimensional (3-D) morphological features are illuminated throughout the development. We provide evidence that the dorsal peri-cloacal mesenchyme (dPCM) remains stationary while other surrounding tissues grow towards it. This causes dramatic changes of spatial relationship among caudal structures and morphological transformation of the cloaca. The 3-D characterizations of Dkk1 mutants reveal a hyperplastic defect of dPCM, which leads to a significant anterior shift of the caudal boundary of the cloaca, premature occlusion of the cloaca and, imperforate anus phenotype. Conversely, Shh knockout causes a severe hypoplastic defect of cloaca mesenchyme including dPCM and persistent cloaca. Collectively, these findings suggest that formation of the dPCM is critical for cloacal morphogenesis and furthermore, growth and movement of the mesenchymal tissues towards the dPCM lead to the cloaca occlusion and separation of the urinary and digestive outlets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.