ArticlePloS one2015
Congo Red Interactions with Curli-Producing E. coli and Native Curli Amyloid Fibers.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A Novel Biofilm Amyloid Ligand for Caspase-11 Drives Hyperactivation in Dendritic Cells.bioRxiv : the preprint server for biology · 2026Article
- Complementary functionalities of extracellular polymeric substances, adhesion ability and hydrophobicity in Pseudomonas isolates may help the selection of strategically advantageous microbial inoculants.World journal of microbiology & biotechnology · 2026Article
- Identification of host genetic factors modulating β-lactam resistance inEmerging microbes & infections · 2025Article
- Serrapeptase EliminatesMicroorganisms · 2025Article
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- Quantitative detection of amyloid fibrils using fluorescence resonance energy transfer between engineered yellow and cyan proteins.Protein science : a publication of the Protein Society · 2025Article
- Diverse polysaccharide production and biofilm formation abilities of clinical Klebsiella pneumoniae.NPJ biofilms and microbiomes · 2024Article
- Antibiofilm Analysis, Synergistic Potential and Biocompatibility Evaluation of a Bacteriocin fromIndian journal of microbiology · 2024Article
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- Synthesis of a Phosphoethanolamine Cellulose Mimetic and Evaluation of Its Unanticipated Biofilm Modulating Properties.ACS infectious diseases · 2024Article
- Diverse polysaccharide production and biofilm formation abilities of clinical Klebsiella pneumoniae.Research square · 2024Article
- Oleanolic Acid Promotes the Formation of ProbioticMicroorganisms · 2024Article
- Article
- In vitro and in silico evaluation of the serrapeptase effect on biofilm and amyloids of Pseudomonas aeruginosa.Applied microbiology and biotechnology · 2023Article
- Characterization of functional amyloid curli in biofilm formation of an environmental isolate Enterobacter cloacae SBP-8.Antonie van Leeuwenhoek · 2023Article
- Biofilm Formation by Mutant Strains of Bacilli under Different Stress Conditions.Microorganisms · 2023Article
- Serrapeptase impairs biofilm, wall, and phospho-homeostasis of resistant and susceptible Staphylococcus aureus.Applied microbiology and biotechnology · 2023Article
- Comparative genomic analysis of uropathogenicFrontiers in microbiology · 2023Article
- Geno- and Phenotypic Characteristics of aMicroorganisms · 2022Article
- A Genetically Engineered Biofilm Material for SARS-CoV-2 Capturing and Isolation.Advanced materials interfaces · 2022Article
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7 authors.
Funding
Abstract
Microorganisms produce functional amyloids that can be examined and manipulated in vivo and in vitro. Escherichia coli assemble extracellular adhesive amyloid fibers termed curli that mediate adhesion and promote biofilm formation. We have characterized the dye binding properties of the hallmark amyloid dye, Congo red, with curliated E. coli and with isolated curli fibers. Congo red binds to curliated whole cells, does not inhibit growth, and can be used to comparatively quantify whole-cell curliation. Using Surface Plasmon Resonance, we measured the binding and dissociation kinetics of Congo red to curli. Furthermore, we determined that the binding of Congo red to curli is pH-dependent and that histidine residues in the CsgA protein do not influence Congo red binding. Our results on E. coli strain MC4100, the most commonly employed strain for studies of E. coli amyloid biogenesis, provide a starting point from which to compare the influence of Congo red binding in other E. coli strains and amyloid-producing organisms.
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