ArticlePLoS neglected tropical diseases2015
Development of a Luminex Bead Based Assay for Diagnosis of Toxocariasis Using Recombinant Antigens Tc-CTL-1 and Tc-TES-26.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Human toxocariasis.Clinical microbiology reviews · 2025Pooled it
- Comparison of Antigen Conjugation to a Peptidic Carrier or to Bovine Serum Albumin in the Serodiagnosis of Canine Visceral Leishmaniasis via Suspension Array Technology.Antibodies (Basel, Switzerland) · 2025Article
- Testing for Antibodies to Four Parasites in Residual Blood Specimens from Trachoma Surveys in Kiribati, 2015-2019.The American journal of tropical medicine and hygiene · 2025Article
- In Silico Discovery of Antigenic-Secreted Proteins to Diagnostic Human Toxocariasis.Acta parasitologica · 2025Article
- Cloning, bioinformatics analysis, and expression of the ubiquitin 2 (ubq-2) gene from the dog roundwormFrontiers in veterinary science · 2025Article
- Immunogenic mapping of potential epitopes from Tc-CTL-1 for the diagnosis of murine toxocariasis.Memorias do Instituto Oswaldo Cruz · 2025Article
- Serological diagnosis ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Cross-Sectional Study of Soil-Transmitted Helminthiases in Black Belt Region of Alabama, USA.Emerging infectious diseases · 2023Article
- Association of infection withJournal of epidemiology and community health · 2023Article
- Article
- Isolation and Production of Human Monoclonal Antibody Proteins against aPathogens (Basel, Switzerland) · 2022Article
- Evaluation of newHeliyon · 2022Article
- Multiple-bead assay for the differential serodiagnosis of neglected human cestodiases: Neurocysticercosis and cystic echinococcosis.PLoS neglected tropical diseases · 2022Article
- Cross-sectional association of Toxoplasma gondii exposure with BMI and diet in US adults.PLoS neglected tropical diseases · 2021Article
- Parasitic Infection Surveillance in Mississippi Delta Children.The American journal of tropical medicine and hygiene · 2020Article
- Rapid, multiplexed detection of biomolecules using electrically distinct hydrogel beads.Lab on a chip · 2020Article
- Seroprevalence of Larval Toxocarosis in the Czech Republic.Acta parasitologica · 2020Article
- Parasitic infections represent a significant health threat among recent immigrants in Chicago.Parasitology research · 2020Article
- Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins.Nature communications · 2018Article
- Seroprevalence of Antibodies to Toxocara Species in the United States and Associated Risk Factors, 2011-2014.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
The clinical spectrum of human disease caused by the roundworms Toxocara canis and Toxocara cati ranges from visceral and ocular larva migrans to covert toxocariasis. The parasite is not typically recovered in affected tissues, so detection of parasite-specific antibodies is usually necessary for establishing a diagnosis. The most reliable immunodiagnostic methods use the Toxocara excretory-secretory antigens (TES-Ag) in ELISA formats to detect Toxocara-specific antibodies. To eliminate the need for native parasite materials, we identified and purified immunodiagnostic antigens using 2D gel electrophoresis followed by electrospray ionization mass spectrometry. Three predominant immunoreactive proteins were found in the TES; all three had been previously described in the literature: Tc-CTL-1, Tc-TES-26, and Tc-MUC-3. We generated Escherichia coli expressed recombinant proteins for evaluation in Luminex based immunoassays. We were unable to produce a functional assay with the Tc-MUC-3 recombinant protein. Tc-CTL-1 and Tc-TES-26 were successfully coupled and tested using defined serum batteries. The use of both proteins together generated better results than if the proteins were used individually. The sensitivity and specificity of the assay for detecting visceral larval migrans using Tc-CTL-1 plus Tc-TES-26 was 99% and 94%, respectively; the sensitivity for detecting ocular larval migrans was 64%. The combined performance of the new assay was superior to the currently available EIA and could potentially be employed to replace current assays that rely on native TES-Ag.
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