ArticleFrontiers in plant science2015
RNAi-based functional elucidation of PtrPRP, a gene encoding a hybrid proline rich protein, in cold tolerance of Poncirus trifoliata.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 26 citations in OpenAlex.
- Exogenous Proline Maintains Cell Wall Structure and Membrane Integrity in Rice Seedlings Under Cr(VI) Stress Associated with Regulation of Proline-Rich Proteins.International journal of molecular sciences · 2026Article
- Transcriptome and Biochemical Analysis of the Mechanism of Low-Temperature Germination inInternational journal of molecular sciences · 2025Article
- Flavonoids as key players in cold tolerance: molecular insights and applications in horticultural crops.Horticulture research · 2025Article
- Uncovering the molecular mechanisms of Acer fabri in adjusting to low-temperature stress through integrated physiological and transcriptomic analysis.Scientific reports · 2025Article
- Combined Physiological and Transcriptomic Analysis Reveals Key Regulatory Networks and Potential Hub Genes Controlling Chilling Tolerance During Soybean Germination.Plant direct · 2024Article
- Transcriptomic Analysis Reveals Potential Gene Regulatory Networks Under Cold Stress of Loquat (Frontiers in plant science · 2022Article
- Identification and analysis of proline-rich proteins and hybrid proline-rich proteins super family genes fromFrontiers in plant science · 2022Article
- Genome-Wide Identification of PRP Genes in Apple Genome and the Role ofInternational journal of molecular sciences · 2021Article
- BSR-Seq analysis provides insights into the cold stress response of Actinidia arguta F1 populations.BMC genomics · 2021Article
- A chromosome-scale reference genome of trifoliate orange (Poncirus trifoliata) provides insights into disease resistance, cold tolerance and genome evolution in Citrus.The Plant journal : for cell and molecular biology · 2020Article
- Multigene CRISPR/Cas9 genome editing of hybrid proline rich proteins (HyPRPs) for sustainable multi-stress tolerance in crops: the review of a promising approach.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Review
- Genome-Wide Analysis and Expression Profiling of Rice Hybrid Proline-Rich Proteins in Response to Biotic and Abiotic Stresses, and Hormone Treatment.Plants (Basel, Switzerland) · 2019Article
- Pigeonpea Hybrid-Proline-Rich Protein (CcHyPRP) Confers Biotic and Abiotic Stress Tolerance in Transgenic Rice.Frontiers in plant science · 2015Article
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3 authors at 2 institutions in 1 country.
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Abstract
Hybrid proline-rich proteins (HyPRPs) have been suggested to play important roles in various plant development and stress response. In this study, we report the cloning and functional analysis of PtrPRP, a HyPRP-encoding gene of Poncirus trifoliata. PtrPRP contains 176 amino acids, among which 21% are proline residues, and has an 8-cysteine motif (8 CM) domain at the C terminal, a signal peptide and a proline-rich region at the N terminal. PtrPRP is constitutively expressed in root, stem and leaf, with the highest expression levels in leaf. It was progressively induced by cold, but transiently upregulated by salt and ABA. Transgenic P. trifoliata plants with knock-down PtrPRP by RNA interference (RNAi) were generated to investigate the role of PtrPRP in cold tolerance. When challenged by low temperature, the PtrPRP-RNAi plants displayed more sensitive performance compared with wild type (WT), as shown by higher electrolyte leakage and malondialdehyde content. In addition, the RNAi lines accumulated more reactive oxygen species (ROS) and lower levels of proline relative to WT. These results suggested that PtrPRP might be positively involved in cold tolerance by maintaining membrane integrity and ROS homeostasis.
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