ArticleFrontiers in plant science2015
Molecular cloning of AtRS4, a seed specific multifunctional RFO synthase/galactosylhydrolase in Arabidopsis thaliana.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- ZmAGA1 enhances seed germination through raffinose hydrolysis and ZmSnRK1α-mediated starch mobilization in maize.Genome biology · 2026Article
- Reconstruction of the raffinose family oligosaccharide pathway in maize improves heat stress tolerance.Stress biology · 2026Article
- Fine scale gene expression dynamics at the foliar level reveal grapevine strategies to cope with recurrent drought stress.BMC plant biology · 2026Article
- Genome-wide identification of raffinose synthase gene family in barley (Frontiers in plant science · 2026Article
- Elucidating Continental-Wide Phylogeographic and Adaptive Processes Shaping the Genome-Wide Diversity of North America's Most Widely Distributed Tree.Molecular ecology · 2025Article
- Significance of Raffinose Family Oligosaccharides (RFOs) metabolism in plants.Advanced biotechnology · 2024Review
- Low molecular weight carbohydrates and abiotic stress tolerance in lentil (Frontiers in plant science · 2024Review
- Genome-wide characterization of DcHsp90 gene family in carnation (Dianthus caryophyllus L.) and functional analysis of DcHsp90-6 in heat tolerance.Protoplasma · 2023Article
- An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity.The Plant journal : for cell and molecular biology · 2023Article
- Identification, Characterization and Expression Profiling of the RS Gene Family during the Withering Process of White Tea in the Tea Plant (International journal of molecular sciences · 2022Article
- Genome-Wide Expression Profiling Analysis of KiwifruitInternational journal of molecular sciences · 2022Article
- Transcriptional regulation of the raffinose family oligosaccharides pathway inFrontiers in plant science · 2022Article
- Identification and Expression Analysis of the Genes Involved in the Raffinose Family Oligosaccharides Pathway ofPlants (Basel, Switzerland) · 2021Article
- Genome-wide identification and expression analysis of Raffinose synthetase family in cotton.BMC bioinformatics · 2021Article
- Raffinose synthase enhances drought tolerance through raffinose synthesis or galactinol hydrolysis in maize andThe Journal of biological chemistry · 2020Article
- Biosynthesis of Raffinose and Stachyose from Sucrose via anApplied and environmental microbiology · 2019Article
- Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame.Scientific reports · 2018Article
- Genetic Architecture of Dietary Fiber and Oligosaccharide Content in a Middle American Panel of Edible Dry Bean.The plant genome · 2018Article
- Sugar metabolism reprogramming in a non-climacteric bud mutant of a climacteric plum fruit during development on the tree.Journal of experimental botany · 2017Article
- Raffinose Family Oligosaccharides Act As Galactose Stores in Seeds and Are Required for Rapid Germination of Arabidopsis in the Dark.Frontiers in plant science · 2016Article
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3 authors at 1 institution in 1 country.
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Abstract
Stachyose is among the raffinose family oligosaccharides (RFOs) one of the major water-soluble carbohydrates next to sucrose in seeds of a number of plant species. Especially in leguminous seeds, e.g. chickpea, stachyose is reported as the major component. In contrast to their ambiguous potential as essential source of carbon for germination, RFOs are indigestible for humans and can contribute to diverse abdominal disorders. In the genome of Arabidopsis thaliana, six putative raffinose synthase genes are reported, whereas little is known about these putative raffinose synthases and their biochemical characteristics or their contribution to the RFO physiology in A. thaliana. In this paper, we report on the molecular cloning, functional expression in Escherichia coli and purification of recombinant AtRS4 from A. thaliana and the biochemical characterisation of the putative stachyose synthase (AtSTS, At4g01970) as a raffinose and high affinity stachyose synthase (Km for raffinose 259.2 ± 21.15 μM) as well as stachyose and galactinol specific galactosylhydrolase. A T-DNA insertional mutant in the AtRS4 gene was isolated. Only semi-quantitative PCR from WT siliques showed a specific transcriptional AtRS4 PCR product. Metabolite measurements in seeds of ΔAtRS4 mutant plants revealed a total loss of stachyose in ΔAtRS4 mutant seeds. We conclude that AtRS4 is the only stachyose synthase in the genome of A. thaliana that AtRS4 represents a key regulation mechanism in the RFO physiology of A. thaliana due to its multifunctional enzyme activity and that AtRS4 is possibly the second seed specific raffinose synthase beside AtRS5, which is responsible for Raf accumulation under abiotic stress.
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