ArticleThe Journal of cell biology2015
Molecular control of irreversible bistability during trypanosome developmental commitment.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Distinguishing functions of trypanosomatid protein kinases.Trends in parasitology · 2022Review
- Article
- The genomic basis of host and vector specificity in non-pathogenic trypanosomatids.Biology open · 2022Article
- NNature · 2022Article
- Insights into the functions and RNA binding ofParasitology · 2021Article
- Right place, right time: Environmental sensing and signal transduction directs cellular differentiation and motility in Trypanosoma brucei.Molecular microbiology · 2021Review
- Basement membrane proteins as a substrate for efficient Trypanosoma brucei differentiation in vitro.PLoS neglected tropical diseases · 2021Article
- An Alba-domain protein required for proteome remodelling during trypanosome differentiation and host transition.PLoS pathogens · 2021Article
- Gel-Based Methods for the Investigation of Signal Transduction Pathways in Trypanosoma brucei.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Proteome-Wide Quantitative Phosphoproteomic Analysis of Trypanosoma brucei Insect and Mammalian Life Cycle Stages.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Organising the cell cycle in the absence of transcriptional control: Dynamic phosphorylation co-ordinates the Trypanosoma brucei cell cycle post-transcriptionally.PLoS pathogens · 2019Article
- Article
- A gene expression comparison of Trypanosoma brucei and Trypanosoma congolense in the bloodstream of the mammalian host reveals species-specific adaptations to density-dependent development.PLoS neglected tropical diseases · 2018Article
- Dissipative structures in biological systems: bistability, oscillations, spatial patterns and waves.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2018Review
- Translational repression by an RNA-binding protein promotes differentiation to infective forms in Trypanosoma cruzi.PLoS pathogens · 2018Article
- Inositol polyphosphate multikinase regulation of Trypanosoma brucei life stage development.Molecular biology of the cell · 2018Article
- Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3'UTR.PloS one · 2018Article
- Expression of the RNA-binding protein RBP10 promotes the bloodstream-form differentiation state in Trypanosoma brucei.PLoS pathogens · 2017Article
- An Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theileri.Genome biology and evolution · 2017Article
- The Cytological Events and Molecular Control of Life Cycle Development of Trypanosoma brucei in the Mammalian Bloodstream.Pathogens (Basel, Switzerland) · 2017Review
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Authors and funding
5 authors.
Funding
Abstract
The life cycle of Trypanosoma brucei involves developmental transitions that allow survival, proliferation, and transmission of these parasites. One of these, the differentiation of growth-arrested stumpy forms in the mammalian blood into insect-stage procyclic forms, can be induced synchronously in vitro with cis-aconitate. Here, we show that this transition is an irreversible bistable switch, and we map the point of commitment to differentiation after exposure to cis-aconitate. This irreversibility implies that positive feedback mechanisms operate to allow commitment (i.e., the establishment of "memory" of exposure to the differentiation signal). Using the reversible translational inhibitor cycloheximide, we show that this signal memory requires new protein synthesis. We further performed stable isotope labeling by amino acids in cell culture to analyze synchronized parasite populations, establishing the protein and phosphorylation profile of parasites pre- and postcommitment, thereby defining the "commitment proteome." Functional interrogation of this data set identified Nek-related kinase as the first-discovered protein kinase controlling the initiation of differentiation to procyclic forms.
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