ArticleProceedings of the National Academy of Sciences of the United States of America2015
VEGF-B-Neuropilin-1 signaling is spatiotemporally indispensable for vascular and neuronal development in zebrafish.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- Angiogenesis, signaling pathways, and animal models.Chinese medical journal · 2025Review
- Research Progress of Docetaxel Nano-Drug Delivery System in the Treatment of Breast Cancer.International journal of nanomedicine · 2025Review
- Methylome analysis of endothelial cells suggests new insights on sporadic brain arteriovenous malformation.Heliyon · 2024Article
- Article
- Targeting circular RNA-MET for anti-angiogenesis treatment via inhibiting endothelial tip cell specialization.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Peptides Derived from Vascular Endothelial Growth Factor B Show Potent Binding to Neuropilin-1.Chembiochem : a European journal of chemical biology · 2022Article
- VEGFB Promotes Myoblasts Proliferation and Differentiation through VEGFR1-PI3K/Akt Signaling Pathway.International journal of molecular sciences · 2021Article
- Cerebrovascular development: mechanisms and experimental approaches.Cellular and molecular life sciences : CMLS · 2021Review
- Vascular endothelial growth factor B inhibits insulin secretion in MIN6 cells and reduces CaWorld journal of diabetes · 2021Article
- VEGF-B Is an Autocrine Gliotrophic Factor for Müller Cells under Pathologic Conditions.Investigative ophthalmology & visual science · 2020Article
- Susceptibility to Cardiac Arrhythmias and Sympathetic Nerve Growth in VEGF-B Overexpressing Myocardium.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Modeling ZNF408-Associated FEVR in Zebrafish Results in Abnormal Retinal Vasculature.Investigative ophthalmology & visual science · 2020Article
- Intussusceptive Vascular Remodeling Precedes Pathological Neovascularization.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- De Novo Mutations of CCNK Cause a Syndromic Neurodevelopmental Disorder with Distinctive Facial Dysmorphism.American journal of human genetics · 2018Article
- Vegfaa instructs cardiac muscle hyperplasia in adult zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis.Angiogenesis · 2018Article
- Guidance Molecules in Vascular Smooth Muscle.Frontiers in physiology · 2018Review
- Metformin potentiates anti-tumor effect of resveratrol on pancreatic cancer by down-regulation of VEGF-B signaling pathway.Oncotarget · 2016Article
- Discontinuation of anti-VEGF cancer therapy promotes metastasis through a liver revascularization mechanism.Nature communications · 2016Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physiological functions of vascular endothelial growth factor (VEGF)-B remain an enigma, and deletion of the Vegfb gene in mice lacks an overt phenotype. Here we show that knockdown of Vegfba, but not Vegfbb, in zebrafish embryos by specific morpholinos produced a lethal phenotype owing to vascular and neuronal defects in the brain. Vegfba morpholinos also markedly prevented development of hyaloid vasculatures in the retina, but had little effects on peripheral vascular development. Consistent with phenotypic defects, Vegfba, but not Vegfaa, mRNA was primarily expressed in the brain of developing zebrafish embryos. Interestingly, in situ detection of Neuropilin1 (Nrp1) mRNA showed an overlapping expression pattern with Vegfba, and knockdown of Nrp1 produced a nearly identically lethal phenotype as Vegfba knockdown. Furthermore, zebrafish VEGF-Ba protein directly bound to NRP1. Importantly, gain-of-function by exogenous delivery of mRNAs coding for NRP1-binding ligands VEGF-B or VEGF-A to the zebrafish embryos rescued the lethal phenotype by normalizing vascular development. Similarly, exposure of zebrafish embryos to hypoxia also rescued the Vegfba morpholino-induced vascular defects in the brain by increasing VEGF-A expression. Independent evidence of VEGF-A gain-of-function was provided by using a functionally defective Vhl-mutant zebrafish strain, which again rescued the Vegfba morpholino-induced vascular defects. These findings show that VEGF-B is spatiotemporally required for vascular development in zebrafish embryos and that NRP1, but not VEGFR1, mediates the essential signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.