ArticleInfection and immunity2016
Surface Polysaccharide Mutants Reveal that Absence of O Antigen Reduces Biofilm Formation of Actinobacillus pleuropneumoniae.
Article in Infection and immunity, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Characterization of two exoUBMC microbiology · 2026Article
- The Impact of Antibiofilm Strategies in Controlling Microbial Colonization.Current microbiology · 2025Review
- Genomic and induction evidence for bacteriophage contributions to sargassum-bacteria symbioses.Microbiome · 2024Article
- Influences of flavonoids from Sedum aizoon L. on biofilm formation of Pseudomonas fragi.Applied microbiology and biotechnology · 2023Article
- Characterization of NovelMicrobiology spectrum · 2022Article
- Pernicious Attitude of Microbial Biofilms in Agri-Farm Industries: Acquisitions and Challenges of Existing Antibiofilm Approaches.Microorganisms · 2022Review
- Outer Membrane Vesicles of Actinobacillus pleuropneumoniae Exert Immunomodulatory Effects on Porcine Alveolar Macrophages.Microbiology spectrum · 2022Article
- Genetic Diversity ofVeterinary sciences · 2022Article
- Cytophaga hutchinsonii chu_2177, encoding the O-antigen ligase, is essential for cellulose degradation.Journal of microbiology (Seoul, Korea) · 2022Article
- Insecticidal Serralysin ofFrontiers in microbiology · 2022Article
- Why vary what's working? Phase variation and biofilm formation inFrontiers in microbiology · 2022Review
- The two-component system CpxA/CpxR is critical for full virulence inFrontiers in microbiology · 2022Article
- Article
- Interactions between the Prophage 919TP and ItsViruses · 2021Article
- Outer Membrane Protein F Is Involved in Biofilm Formation, Virulence and Antibiotic Resistance inMicroorganisms · 2021Article
- Quinolone Resistance ofInternational journal of molecular sciences · 2021Article
- Bacterial extracellular matrix as a natural source of biotechnologically multivalent materials.Computational and structural biotechnology journal · 2021Review
- Resistance of Tick Gut Microbiome to Anti-Tick Vaccines, Pathogen Infection and Antimicrobial Peptides.Pathogens (Basel, Switzerland) · 2020Article
- Article
- Helicobacter pylori Biofilm Formation and Its Potential Role in Pathogenesis.Microbiology and molecular biology reviews : MMBR · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Actinobacillus pleuropneumoniae is a Gram-negative bacterium belonging to the Pasteurellaceae family and the causative agent of porcine pleuropneumonia, a highly contagious lung disease causing important economic losses. Surface polysaccharides, including lipopolysaccharides (LPS) and capsular polysaccharides (CPS), are implicated in the adhesion and virulence of A. pleuropneumoniae, but their role in biofilm formation is still unclear. In this study, we investigated the requirement for these surface polysaccharides in biofilm formation by A. pleuropneumoniae serotype 1. Well-characterized mutants were used: an O-antigen LPS mutant, a truncated core LPS mutant with an intact O antigen, a capsule mutant, and a poly-N-acetylglucosamine (PGA) mutant. We compared the amount of biofilm produced by the parental strain and the isogenic mutants using static and dynamic systems. Compared to the findings for the biofilm of the parental or other strains, the biofilm of the O antigen and the PGA mutants was dramatically reduced, and it had less cell-associated PGA. Real-time PCR analyses revealed a significant reduction in the level of pgaA, cpxR, and cpxA mRNA in the biofilm cells of the O-antigen mutant compared to that in the biofilm cells of the parental strain. Specific binding between PGA and LPS was consistently detected by surface plasmon resonance, but the lack of O antigen did not abolish these interactions. In conclusion, the absence of the O antigen reduces the ability of A. pleuropneumoniae to form a biofilm, and this is associated with the reduced expression and production of PGA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.