ArticleJournal of cell science2015
p114RhoGEF governs cell motility and lumen formation during tubulogenesis through a ROCK-myosin-II pathway.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
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- Efficient functional cyst formation of biliary epithelial cells using microwells for potential bile duct organisation in vitro.Scientific reports · 2018Article
- Expression of novel "LOCGEF" isoforms of ARHGEF18 in eosinophils.Journal of leukocyte biology · 2018Article
- Association between a Single Nucleotide Polymorphism in the 3'-UTR ofDisease markers · 2018Article
- Afadin orients cell division to position the tubule lumen in developing renal tubules.Development (Cambridge, England) · 2017Article
- Mapping the chromatin landscape and Blimp1 transcriptional targets that regulate trophoblast differentiation.Scientific reports · 2017Article
- Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration.The Journal of cell biology · 2017Article
- A Gα12-specific Binding Domain in AKAP-Lbc and p114RhoGEF.Journal of molecular signaling · 2016Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Tubulogenesis is fundamental to the development of many epithelial organs. Although lumen formation in cysts has received considerable attention, less is known about lumenogenesis in tubes. Here, we utilized tubulogenesis induced by hepatocyte growth factor (HGF) in MDCK cells, which form tubes enclosing a single lumen. We report the mechanism that controls tubular lumenogenesis and limits each tube to a single lumen. Knockdown of p114RhoGEF (also known as ARHGEF18), a guanine nucleotide exchange factor for RhoA, did not perturb the early stages of tubulogenesis induced by HGF. However, this knockdown impaired later stages of tubulogenesis, resulting in multiple lumens in a tube. Inhibition of Rho kinase (ROCK) or myosin IIA, which are downstream of RhoA, led to formation of multiple lumens. We studied lumen formation by live-cell imaging, which revealed that inhibition of this pathway blocked cell movement, suggesting that cell movement is necessary for consolidating multiple lumens into a single lumen. Lumen formation in tubules is mechanistically quite different from lumenogenesis in cysts. Thus, we demonstrate a new pathway that regulates directed cell migration and formation of a single lumen during epithelial tube morphogenesis.
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