ArticleMolecular microbiology2016
Colony-morphology screening uncovers a role for the Pseudomonas aeruginosa nitrogen-related phosphotransferase system in biofilm formation.
Article in Molecular microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Experimental evolution reveals adaptive pathways to reduced antibiotic susceptibility inMicrobiology (Reading, England) · 2026Article
- Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.Journal of bacteriology · 2026Review
- Quorum sensing regulation by the nitrogen phosphotransferase system inJournal of bacteriology · 2025Article
- Toroidal displacement ofmBio · 2025Article
- EF-hand calcium sensor, EfhP, controls transcriptional regulation of iron uptake by calcium inmBio · 2024Article
- Article
- Population structure and ongoing microevolution of the emerging multidrug-resistant Salmonella Typhimurium ST213.npj antimicrobials and resistance · 2024Article
- Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.PLoS biology · 2024Article
- Machine learning identification of Pseudomonas aeruginosa strains from colony image data.PLoS computational biology · 2023Article
- A putative lipase affectsmSphere · 2023Article
- High-throughput microbial culturomics using automation and machine learning.Nature biotechnology · 2023Article
- Cell arrangement impacts metabolic activity and antibiotic tolerance inbioRxiv : the preprint server for biology · 2023Article
- PtsN in Pseudomonas aeruginosa Is Phosphorylated by Redundant Upstream Proteins and Impacts Virulence-Related Genes.Journal of bacteriology · 2023Article
- Redundancy in Citrate andJournal of bacteriology · 2022Article
- Article
- Clemastine Inhibits the Biofilm and Hemolytic of Staphylococcus aureus through the GdpP Protein.Microbiology spectrum · 2022Article
- Article
- Tobramycin Adaptation Enhances Policing of Social Cheaters in Pseudomonas aeruginosa.Applied and environmental microbiology · 2021Article
- Secondary metabolites from plant-associated Pseudomonas are overproduced in biofilm.Microbial biotechnology · 2020Article
- Elevated exopolysaccharide levels in Pseudomonas aeruginosa flagellar mutants have implications for biofilm growth and chronic infections.PLoS genetics · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Pseudomonas aeruginosa is an opportunistic human pathogen whose survival is aided by forming communities known as biofilms, in which cells are encased in a self-produced matrix. We devised a mutant screen based on colony morphology to identify additional genes with previously unappreciated roles in biofilm formation. Our screen, which identified most known biofilm-related genes, also uncovered PA14_16550 and PA14_69700, deletions of which abrogated and augmented biofilm formation respectively. We also identified ptsP, which encodes enzyme I of the nitrogen-regulated phosphotransferase (PTS(Ntr)) system, as being important for cyclic-di-GMP production and for biofilm formation. Further experiments showed that biofilm formation is hindered in the absence of phosphotransfer through the PTS(Ntr), but only in the presence of enzyme II (PtsN), the putative regulatory module of the PTS(Ntr). These results implicate unphosphorylated PtsN as a negative regulator of biofilm formation and establish one of the first known roles of the PTS(Ntr) in P. aeruginosa.
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