ArticleDevelopment (Cambridge, England)2015
Human stem cells from single blastomeres reveal pathways of embryonic or trophoblast fate specification.
Open access · hybridAbstract read
In one paragraph
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41citing papers in PubMed
4.2field-weighted citation impact, top 5% of its field
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Who cites it
41 citing papers in PubMed, 71 citations in OpenAlex.
- Current Translational 3D In Vitro Models of Human Placental Tissue.Stem cell reviews and reports · 2026Review
- Multi-Omics Reveals Early Pregnancy Placental Dysfunction Associated With Preterm and Term Preeclampsia.MedComm · 2026Article
- Modeling Early Human Embryo Implantation Using Human Endometrial Organoids and Embryo Surrogate Co-cultures for microRNA Transfection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- WD-repeat containing protein-61 regulates endometrial epithelial cell adhesion indicating an important role in receptivity.Molecular human reproduction · 2024Article
- Deciphering the Epigenetic Landscape: Placental Development and Its Role in Pregnancy Outcomes.Stem cell reviews and reports · 2024Review
- Extracellular vesicles affecting embryo developmentFrontiers in pharmacology · 2024Review
- Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Modeling the human placental barrier to understandFrontiers in cellular and infection microbiology · 2023Review
- Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures.Frontiers in cell and developmental biology · 2023Article
- Activation of embryonic/germ cell-like axis links poor outcomes of gliomas.Cancer cell international · 2022Article
- New insights into the epitranscriptomic control of pluripotent stem cell fate.Experimental & molecular medicine · 2022Review
- Modelling human placental villous development: designing cultures that reflect anatomy.Cellular and molecular life sciences : CMLS · 2022Review
- Polyploid giant cancer cells are dependent on cholesterol for progeny formation through amitotic division.Scientific reports · 2022Article
- In Vitro Model of Human Trophoblast in Early Placentation.Biomedicines · 2022Review
- Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape.Frontiers in cell and developmental biology · 2022Article
- Infertile human endometrial organoid apical protein secretions are dysregulated and impair trophoblast progenitor cell adhesion.Frontiers in endocrinology · 2022Article
- Contiguous erosion of the inactive X in human pluripotency concludes with global DNA hypomethylation.Cell reports · 2021Article
- In vitro models of the human endometrium: evolution and application for women's health.Biology of reproduction · 2021Review
- Unique features and emerging in vitro models of human placental development.Reproductive medicine and biology · 2020Review
- Side-Population Trophoblasts Exhibit the Differentiation Potential of a Trophoblast Stem Cell Population, Persist to Term, and are Reduced in Fetal Growth Restriction.Stem cell reviews and reports · 2020Article
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Authors and funding
20 authors at 6 institutions in 2 countries.
Tamara ZdravkovicCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA.
Kristopher L NazorCenter for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Nicholas LarocqueCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA.
Matthew GormleyCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA.
Matthew DonneCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA.
Nathan HunkapillarCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA.
Gnanaratnam GiritharanStemLifeLine, San Carlos, CA 94070, USA.
Harold S BernsteinThe Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Department of Pediatrics, University of California San Francisco, San Francisco, CA 94143, USA.
Grace WeiThe Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Diabetes Center, Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.
Matthias HebrokDiabetes Center, Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.
Xianmin ZengBuck Institute for Research on Aging, Novato, CA 94945, USA.
Olga GenbacevCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA.
Aras MattisThe Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Department of Pathology, University of California San Francisco, San Francisco, CA 94143, USA.
Michael T McMasterThe Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA 94143, USA.
Ana KrtolicaStemLifeLine, San Carlos, CA 94070, USA.
Diana ValbuenaFundación Instituto Valenciano de Infertilidad (IVI), Parc Científic Universitat de València, 46980, Valencia, Spain.
Carlos SimónFundación Instituto Valenciano de Infertilidad (IVI), Parc Científic Universitat de València, 46980, Valencia, Spain.
Louise C LaurentCenter for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA Department of Reproductive Medicine, University of California San Diego, La Jolla, CA 92093, USA.
Jeanne F LoringCenter for Regenerative Medicine, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Susan J FisherCenter for Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA Division of Maternal Fetal Medicine, University of California San Francisco, San Francisco, CA 94143, USA Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California San Francisco, San Francisco, CA 94143, USA The Eli & Edythe Broad Center for Regeneration Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA 94143, USA Human Embryonic Stem Cell Program, University of California San Francisco, San Francisco, CA 94143, USA Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA sfisher@cgl.ucsf.edu.
Broad Center · USUniversity of California, San Francisco · USScripps Research Institute · USUniversitat de València · ESBuck Institute for Research on Aging · USReproductive Science Center · US
Funding
Research BaseP30DK063720 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GERMAN, MICHAEL S · 2003 to 2019
$21.8MRegulation of Lipid Metabolism by miR-29a within HepatocytesK08DK098270 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MATTIS, ARAS NIKODEMAS · 2013 to 2017
$789kNIDDK NIH HHS K08 DK098270NIDDK NIH HHS P30 DK063720
Abstract
Mechanisms of initial cell fate decisions differ among species. To gain insights into lineage allocation in humans, we derived ten human embryonic stem cell lines (designated UCSFB1-10) from single blastomeres of four 8-cell embryos and one 12-cell embryo from a single couple. Compared with numerous conventional lines from blastocysts, they had unique gene expression and DNA methylation patterns that were, in part, indicative of trophoblast competence. At a transcriptional level, UCSFB lines from different embryos were often more closely related than those from the same embryo. As predicted by the transcriptomic data, immunolocalization of EOMES, T brachyury, GDF15 and active β-catenin revealed differential expression among blastomeres of 8- to 10-cell human embryos. The UCSFB lines formed derivatives of the three germ layers and CDX2-positive progeny, from which we derived the first human trophoblast stem cell line. Our data suggest heterogeneity among early-stage blastomeres and that the UCSFB lines have unique properties, indicative of a more immature state than conventional lines.
Indexed as
Embryo Culture Techniquesbeta CateninBlastocystBlastomeresCell DifferentiationCell LineCell LineageDNA MethylationEmbryonic Stem CellsEndodermFibroblastsGene Expression ProfilingGene Expression Regulation, DevelopmentalGrowth Differentiation Factor 15HumansMyocytes, Cardiacbeta CateninCTNNB1 protein, humanGDF15 protein, humanGrowth Differentiation Factor 15BlastomereEpigenomeFate specificationHuman embryoHuman embryonic stem cellHuman trophoblast stem cellTranscriptome
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.