ArticleAnalytical and bioanalytical chemistry2016
Digital PCR for direct quantification of viruses without DNA extraction.
Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- A novel five-plex digital PCR assay for the simultaneous detection of murine pathogens: Sendai virus, reovirus, mouse parvoviruses, pneumonia virus of mice, and mouse hepatitis virus.Microbiology spectrum · 2026Article
- Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.Veterinary medicine and science · 2026Article
- Rapid and Sensitive Digital Droplet PCR Assays for Detecting HPV16 DNA in Liquid Biopsies.Journal of medical virology · 2025Article
- Identification ofInfection and drug resistance · 2024Review
- Improvement of Sensitivity and Speed of Virus Sensing Technologies Using nm- and μm-Scale Components.Sensors (Basel, Switzerland) · 2023Review
- Hydrogel capsule-based digital quantitative polymerase chain reaction.Mikrochimica acta · 2023Article
- Evaluation of DNA Extraction Methods for Reliable Quantification ofBiosensors · 2023Article
- Metrological framework to support accurate, reliable, and reproducible nucleic acid measurements.Analytical and bioanalytical chemistry · 2022Review
- Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction.Analytical chemistry · 2021Article
- Droplet digital PCR of viral DNA/RNA, current progress, challenges, and future perspectives.Journal of medical virology · 2021Review
- Comprehensive characterization and quantification of adeno associated vectors by size exclusion chromatography and multi angle light scattering.Scientific reports · 2021Article
- Accurate bulk quantitation of droplet digital PCR.bioRxiv : the preprint server for biology · 2021Article
- Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR.Scientific reports · 2020Article
- Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples.Frontiers in veterinary science · 2020Article
- Accurate Quantification and Characterization of Adeno-Associated Viral Vectors.Frontiers in microbiology · 2019Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Development of sensitive ddPCR assays to reliably quantify the proviral DNA reservoir in all common circulating HIV subtypes and recombinant forms.Journal of the International AIDS Society · 2018Article
- Applying Quantitative Molecular Tools for Virus Transport Studies: Opportunities and Challenges.Ground water · 2017Article
- Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR.Analytical and bioanalytical chemistry · 2017Article
- Applications of Digital PCR for Clinical Microbiology.Journal of clinical microbiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA extraction before amplification is considered an essential step for quantification of viral DNA using real-time PCR (qPCR). However, this can directly affect the final measurements due to variable DNA yields and removal of inhibitors, which leads to increased inter-laboratory variability of qPCR measurements and reduced agreement on viral loads. Digital PCR (dPCR) might be an advantageous methodology for the measurement of virus concentrations, as it does not depend on any calibration material and it has higher tolerance to inhibitors. DNA quantification without an extraction step (i.e. direct quantification) was performed here using dPCR and two different human cytomegalovirus whole-virus materials. Two dPCR platforms were used for this direct quantification of the viral DNA, and these were compared with quantification of the extracted viral DNA in terms of yield and variability. Direct quantification of both whole-virus materials present in simple matrices like cell lysate or Tris-HCl buffer provided repeatable measurements of virus concentrations that were probably in closer agreement with the actual viral load than when estimated through quantification of the extracted DNA. Direct dPCR quantification of other viruses, reference materials and clinically relevant matrices is now needed to show the full versatility of this very promising and cost-efficient development in virus quantification.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.