ArticleGenetics2015
Regulation of CTP Synthase Filament Formation During DNA Endoreplication in Drosophila.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- Monomethylation of cytosolic 5'-nucleotidase 1A affects its recognition by inclusion body myositis autoantibodies.Journal of neuromuscular diseases · 2026Article
- Integrative role of CTPS cytoophidia in polyploid tissue growth and nutrient adaptation.Insect science · 2026Article
- The Impact of Developmental and Metabolic Cues on Cytoophidium Formation.International journal of molecular sciences · 2024Review
- Light-sensitive phosphorylation regulates retinal IMPDH1 activity and filament assembly.The Journal of cell biology · 2024Article
- Connecting Hippo Pathway and Cytoophidia inInternational journal of molecular sciences · 2024Article
- Article
- Structural basis of human PRPS2 filaments.Cell & bioscience · 2023Article
- Greater than the sum of parts: Mechanisms of metabolic regulation by enzyme filaments.Current opinion in structural biology · 2023Review
- CTP synthase: the hissing of the cellular serpent.Histochemistry and cell biology · 2022Review
- Article
- GTP-Dependent Regulation of CTP Synthase: Evolving Insights into Allosteric Activation and NHBiomolecules · 2022Review
- CTP synthase polymerization in germline cells of the developingBiology open · 2020Article
- Freedom of assembly: metabolic enzymes come together.Molecular biology of the cell · 2020Article
- mTOR-S6K1 pathway mediates cytoophidium assembly.Journal of genetics and genomics = Yi chuan xue bao · 2019Article
- Forming cytoophidia prolongs the half-life of CTP synthase.Cell discovery · 2019Article
- Interfilament interaction between IMPDH and CTPS cytoophidia.The FEBS journal · 2018Article
- Transcriptome Analysis of Intrusively Growing Flax Fibers Isolated by Laser Microdissection.Scientific reports · 2018Article
- Spatial Organization of Metabolic Enzyme Complexes in Cells.Biochemistry · 2017Review
- Critical roles of CTP synthase N-terminal in cytoophidium assembly.Experimental cell research · 2017Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CTP synthase (CTPsyn) plays an essential role in DNA, RNA, and lipid synthesis. Recent studies in bacteria, yeast, and Drosophila all reveal a polymeric CTPsyn structure, which dynamically regulates its enzymatic activity. However, the molecular mechanism underlying the formation of CTPsyn polymers is not completely understood. In this study, we found that reversible ubiquitination regulates the dynamic assembly of the filamentous structures of Drosophila CTPsyn. We further determined that the proto-oncogene Cbl, an E3 ubiquitin ligase, controls CTPsyn filament formation in endocycles. While the E3 ligase activity of Cbl is required for CTPsyn filament formation, Cbl does not affect the protein levels of CTPsyn. It remains unclear whether the regulation of CTPsyn filaments by Cbl is through direct ubiquitination of CTPsyn. In the absence of Cbl or with knockdown of CTPsyn, the progression of the endocycle-associated S phase was impaired. Furthermore, overexpression of wild-type, but not enzymatically inactive CTPsyn, rescued the endocycle defect in Cbl mutant cells. Together, these results suggest that Cbl influences the nucleotide pool balance and controls CTPsyn filament formation in endocycles. This study links Cbl-mediated ubiquitination to the polymerization of a metabolic enzyme and reveals a role for Cbl in endocycles during Drosophila development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.