ArticleGenetics2015
Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- CRISPR/Cas9 as a Mutagenic Factor.International journal of molecular sciences · 2024Review
- CRISPR-mediated genome editing allows for efficient on demand creation of >200 kb deficiencies with precise boundaries.microPublication biology · 2023Article
- A Decade of CRISPR-Cas Gnome Editing inInternational journal of molecular sciences · 2022Review
- Speciation and adaptation research meets genome editing.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Bisection of the X chromosome disrupts the initiation of chromosome silencing during meiosis in Caenorhabditis elegans.Nature communications · 2021Article
- Review
- Aspects of Gene Therapy Products Using Current Genome-Editing Technology in Japan.Human gene therapy · 2020Review
- In vivo CRISPR screening for phenotypic targets of theGenes & development · 2020Article
- Peel-1 negative selection promotes screening-free CRISPR-Cas9 genome editing in Caenorhabditis elegans.PloS one · 2020Article
- Targeted Chromosomal Rearrangements via Combinatorial Use of CRISPR/Cas9 and Cre/G3 (Bethesda, Md.) · 2018Article
- Combined flow cytometry and high-throughput image analysis for the study of essential genes in Caenorhabditis elegans.BMC biology · 2018Article
- CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities.Frontiers in genetics · 2018Review
- Induction of Chromosomal Translocations with CRISPR-Cas9 and Other Nucleases: Understanding the Repair Mechanisms That Give Rise to Translocations.Advances in experimental medicine and biology · 2018Review
- A New Diagnostic Resource forG3 (Bethesda, Md.) · 2017Article
- A Chromosome-Scale Assembly of theG3 (Bethesda, Md.) · 2017Article
- OGEE v2: an update of the online gene essentiality database with special focus on differentially essential genes in human cancer cell lines.Nucleic acids research · 2017Article
- The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models.Dong wu xue yan jiu = Zoological research · 2016Review
- The expanding footprint of CRISPR/Cas9 in the plant sciences.Plant cell reports · 2016Review
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many genes play essential roles in development and fertility; their disruption leads to growth arrest or sterility. Genetic balancers have been widely used to study essential genes in many organisms. However, it is technically challenging and laborious to generate and maintain the loss-of-function mutations of essential genes. The CRISPR/Cas9 technology has been successfully applied for gene editing and chromosome engineering. Here, we have developed a method to induce chromosomal translocations and produce genetic balancers using the CRISPR/Cas9 technology and have applied this approach to edit essential genes in Caenorhabditis elegans. The co-injection of dual small guide RNA targeting genes on different chromosomes resulted in reciprocal translocation between nonhomologous chromosomes. These animals with chromosomal translocations were subsequently crossed with animals that contain normal sets of chromosomes. The F1 progeny were subjected to a second round of Cas9-mediated gene editing. Through this method, we successfully produced nematode strains with specified chromosomal translocations and generated a number of loss-of-function alleles of two essential genes (csr-1 and mes-6). Therefore, our method provides an easy and efficient approach to generate and maintain loss-of-function alleles of essential genes with detailed genetic background information.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.