ArticleGenetics2015
Paramutation in Drosophila Requires Both Nuclear and Cytoplasmic Actors of the piRNA Pathway and Induces Cis-spreading of piRNA Production.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 54 citations in OpenAlex.
- Adaptive P-element Insertions in a Long Non-Coding RNA are Potential Emerging piRNA Clusters.Genome biology and evolution · 2026Article
- Paramutation-Like Behavior of Genic piRNA-Producing Loci inInternational journal of molecular sciences · 2025Article
- Polycomb-mediated transgenerational epigenetic inheritance ofOpen biology · 2025Article
- Article
- Experimentally evolvingGenome research · 2024Article
- Spoink, a LTR retrotransposon, invaded D. melanogaster populations in the 1990s.PLoS genetics · 2024Article
- Article
- Maternally inherited siRNAs initiate piRNA cluster formation.Molecular cell · 2023Article
- The composition of piRNA clusters in Drosophila melanogaster deviates from expectations under the trap model.BMC biology · 2023Article
- Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity.BMC biology · 2023Article
- The histone demethylase Kdm3 prevents auto-immune piRNAs production inScience advances · 2023Article
- Article
- The Transposition Rate Has Little Influence on the Plateauing Level of the P-element.Molecular biology and evolution · 2022Article
- Molecular mechanisms of transgenerational epigenetic inheritance.Nature reviews. Genetics · 2022Review
- Review
- Review
- Article
- Maternally inherited piRNAs direct transient heterochromatin formation at active transposons during earlyeLife · 2021Article
- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
- Germ Granules Allow Transmission of Small RNA-Based Parental Responses in the "Germ Plasm".iScience · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposable element activity is repressed in the germline in animals by PIWI-interacting RNAs (piRNAs), a class of small RNAs produced by genomic loci mostly composed of TE sequences. The mechanism of induction of piRNA production by these loci is still enigmatic. We have shown that, in Drosophila melanogaster, a cluster of tandemly repeated P-lacZ-white transgenes can be activated for piRNA production by maternal inheritance of a cytoplasm containing homologous piRNAs. This activated state is stably transmitted over generations and allows trans-silencing of a homologous transgenic target in the female germline. Such an epigenetic conversion displays the functional characteristics of a paramutation, i.e., a heritable epigenetic modification of one allele by the other. We report here that piRNA production and trans-silencing capacities of the paramutated cluster depend on the function of the rhino, cutoff, and zucchini genes involved in primary piRNA biogenesis in the germline, as well as on that of the aubergine gene implicated in the ping-pong piRNA amplification step. The 21-nt RNAs, which are produced by the paramutated cluster, in addition to 23- to 28-nt piRNAs are not necessary for paramutation to occur. Production of these 21-nt RNAs requires Dicer-2 but also all the piRNA genes tested. Moreover, cytoplasmic transmission of piRNAs homologous to only a subregion of the transgenic locus can generate a strong paramutated locus that produces piRNAs along the whole length of the transgenes. Finally, we observed that maternally inherited transgenic small RNAs can also impact transgene expression in the soma. In conclusion, paramutation involves both nuclear (Rhino, Cutoff) and cytoplasmic (Aubergine, Zucchini) actors of the piRNA pathway. In addition, since it is observed between nonfully homologous loci located on different chromosomes, paramutation may play a crucial role in epigenome shaping in Drosophila natural populations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.