ArticlePlant molecular biology2016
Identification of a regulatory element responsible for salt induction of rice OsRAV2 through ex situ and in situ promoter analysis.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64 citing papers in PubMed, 201 citations in OpenAlex.
- Genome-Wide Identification, Expression and Tissue-Specific Epigenetic Modification Analysis of theBiology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Transcription factor-promoter interactions forbioRxiv : the preprint server for biology · 2025Article
- Genome-wide identification, phylogenetic analysis and expression pattern study of trihelix transcription factors in Pisum sativum L.Scientific reports · 2025Article
- CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.3 Biotech · 2025Review
- Salinity Stress in Rice: Multilayered Approaches for Sustainable Tolerance.International journal of molecular sciences · 2025Review
- Strategies to develop climate-resilient chili peppers: transcription factor optimization through genome editing.Planta · 2025Review
- Microbiome Engineering for Sustainable Rice Production: Strategies for Biofertilization, Stress Tolerance, and Climate Resilience.Microorganisms · 2025Review
- Article
- Promoters, KeyInternational journal of molecular sciences · 2024Review
- Identification and characterization of the Quinoa AP2/ERF gene family and their expression patterns in response to salt stress.Scientific reports · 2024Article
- CRISPR/Cas9 mediated genome editing for crop improvement against Abiotic stresses: current trends and prospects.Functional & integrative genomics · 2024Review
- CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks.Functional & integrative genomics · 2024Review
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
- Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement.GM crops & food · 2023Review
- Genetic Databases and Gene Editing Tools for Enhancing Crop Resistance against Abiotic Stress.Biology · 2023Review
- CRISPR/Cas9-MediatedInternational journal of molecular sciences · 2023Article
- Recent Developments in CRISPR/Cas9 Genome-Editing Technology Related to Plant Disease Resistance and Abiotic Stress Tolerance.Biology · 2023Review
- Genome Editing and Improvement of Abiotic Stress Tolerance in Crop Plants.Life (Basel, Switzerland) · 2023Review
4 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salt is a major environmental stress factor that can affect rice growth and yields. Recent studies suggested that members of the AP2/ERF domain-containing RAV (related to ABI3/VP1) TF family are involved in abiotic stress adaptation. However, the transcriptional response of rice RAV genes (OsRAVs) to salt has not yet been fully characterized. In this study, the expression patterns of all five OsRAVs were examined under salt stress. Only one gene, OsRAV2, was stably induced by high-salinity treatment. Further expression profile analyses indicated that OsRAV2 is transcriptionally regulated by salt, but not KCl, osmotic stress, cold or ABA (abscisic acid) treatment. To elucidate the regulatory mechanism of the stress response at the transcriptional level, we isolated and characterized the promoter region of OsRAV2 (P OsRAV2 ). Transgenic analysis indicated that P OsRAV2 is induced by salt stress but not osmotic stress or ABA treatment. Serial 5' deletions and site-specific mutations in P OsRAV2 revealed that a GT-1 element located at position -664 relative to the putative translation start site is essential for the salt induction of P OsRAV2 . The regulatory function of the GT-1 element in the salt induction of OsRAV2 was verified in situ in plants with targeted mutations generated using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system. Taken together, our results indicate that the GT-1 element directly controls the salt response of OsRAV2. This study provides a better understanding of the putative functions of OsRAVs and the molecular regulatory mechanisms of plant genes under salt stress.
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