Evidence map›Paper›PMID 26481664›Full record

ArticleMolecular cell2015

Anionic Phospholipids Stabilize RecA Filament Bundles in Escherichia coli.

Manohary Rajendram, Leili Zhang, Bradley J Reynolds, George K Auer, Hannah H Tuson, Khanh V Ngo, Michael M Cox, Arun Yethiraj, Qiang Cui, Douglas B Weibel

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 49 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Frontiers in microbiology · 2020
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Cardiolipin AltersmBio · 2019
    Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Manohary RajendramDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Leili ZhangDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Theoretical Chemistry Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
Bradley J ReynoldsDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
George K AuerDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA.
Hannah H TusonDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Khanh V NgoDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Michael M CoxDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Arun YethirajDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Theoretical Chemistry Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
Qiang CuiDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Theoretical Chemistry Institute, University of Wisconsin-Madison, Madison, WI 53706, USA.
Douglas B WeibelDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: weibel@biochem.wisc.edu.
University of Wisconsin–Madison · US

Funding

THE BIOCHEMISTRY OF GENETIC RECOMBINATION/RECA PROTEINR01GM032335 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COX, MICHAEL M. · 1985 to 2014
$6.8M
Revisiting the bacterial cell wall as a target for new antibioticsDP2OD008735 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI WEIBEL, DOUGLAS BENJAMIN · 2011 to 2011
$2.3M
Mechanisms and applications of recombinational DNA repairU01GM032335 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COX, MICHAEL M. · 2015 to 2018
$1.7M
ACQUISITION OF MASS SPECTROMETER FOR CORE FACILITYS10RR013790 · NCRR · UNIVERSITY OF WISCONSIN MADISON · PI RAINES, RONALD T · 1999 to 1999
–
NCRR NIH HHS S10 RR013790NCRR NIH HHS S10 RR13790NIGMS NIH HHS GM32335NIGMS NIH HHS R01 GM032335NIGMS NIH HHS U01 GM032335NIH HHS 1DP2OD008735-01NIH HHS DP2 OD008735
6 · The paper itself

Abstract

We characterize the interaction of RecA with membranes in vivo and in vitro and demonstrate that RecA binds tightly to the anionic phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Using computational models, we identify two regions of RecA that interact with PG and CL: (1) the N-terminal helix and (2) loop L2. Mutating these regions decreased the affinity of RecA to PG and CL in vitro. Using 3D super-resolution microscopy, we demonstrate that depleting Escherichia coli PG and CL altered the localization of RecA foci and hindered the formation of RecA filament bundles. Consequently, E. coli cells lacking aPLs fail to initiate a robust SOS response after DNA damage, indicating that the membrane acts as a scaffold for nucleating the formation of RecA filament bundles and plays an important role in the SOS response.

Indexed as

CardiolipinsCell MembraneDNA, BacterialDNA DamageEscherichia coliEscherichia coli ProteinsPhosphatidylglycerolsProtein Structure, SecondaryProtein Structure, TertiaryRec A RecombinasesSOS Response, GeneticsCardiolipinsDNA, BacterialEscherichia coli ProteinsPhosphatidylglycerolsRec A Recombinases

Identifiers

PMID26481664
PMCPMC4771187
OpenAlexW1904921449

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.