ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 82 citations in OpenAlex.
- Next-generation sequencing: Driving a paradigm shift from pathogen detection to systems-level analysis.Infectious medicine · 2026Article
- Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Autofluorescence lifetime imaging classifies human B and NK cell activation state.Frontiers in bioengineering and biotechnology · 2025Article
- The rise of big data: deep sequencing-driven computational methods are transforming the landscape of synthetic antibody design.Journal of biomedical science · 2024Review
- Early cellular and molecular signatures correlate with severity of West Nile virus infection.iScience · 2023Article
- Review
- Improved computational epitope profiling using structural models identifies a broader diversity of antibodies that bind to the same epitope.Frontiers in molecular biosciences · 2023Article
- Intrathecally Expanding B Cell Clones in Herpes Simplex Encephalitis: A Case Report.Neurology and therapy · 2022Article
- Engineering the Single Domain Antibodies Targeting Receptor Binding Motifs Within the Domain III of West Nile Virus Envelope Glycoprotein.Frontiers in microbiology · 2022Article
- Article
- Harnessing immunological targets for COVID-19 immunotherapy.Future virology · 2021Review
- Alignment free identification of clones in B cell receptor repertoires.Nucleic acids research · 2021Article
- Transcriptome Profiling of the Ovarian Cells at the Single-Cell Resolution in Adult Asian Seabass.Frontiers in cell and developmental biology · 2021Article
- Memory B cell and antibody responses to flavivirus infection and vaccination.Faculty reviews · 2021Review
- Drug Repurposing for Prevention and Treatment of COVID-19: A Clinical Landscape.Discoveries (Craiova, Romania) · 2020Review
- Somatic hypermutation analysis for improved identification of B cell clonal families from next-generation sequencing data.PLoS computational biology · 2020Article
- A Review of SARS-CoV-2 and the Ongoing Clinical Trials.International journal of molecular sciences · 2020Review
- Inferring B cell specificity for vaccines using a Bayesian mixture model.BMC genomics · 2020Article
- VDJbase: an adaptive immune receptor genotype and haplotype database.Nucleic acids research · 2020Article
- Maturation of the Human Immunoglobulin Heavy Chain Repertoire With Age.Frontiers in immunology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 7 institutions in 3 countries.
Funding
Abstract
West Nile virus (WNV) infection is an emerging mosquito-borne disease that can lead to severe neurological illness and currently has no available treatment or vaccine. Using microengraving, an integrated single-cell analysis method, we analyzed a cohort of subjects infected with WNV - recently infected and post-convalescent subjects - and efficiently identified four novel WNV neutralizing antibodies. We also assessed the humoral response to WNV on a single-cell and repertoire level by integrating next generation sequencing (NGS) into our analysis. The results from single-cell analysis indicate persistence of WNV-specific memory B cells and antibody-secreting cells in post-convalescent subjects. These cells exhibited class-switched antibody isotypes. Furthermore, the results suggest that the antibody response itself does not predict the clinical severity of the disease (asymptomatic or symptomatic). Using the nucleotide coding sequences for WNV-specific antibodies derived from single cells, we revealed the ontogeny of expanded WNV-specific clones in the repertoires of recently infected subjects through NGS and bioinformatic analysis. This analysis also indicated that the humoral response to WNV did not depend on an anamnestic response, due to an unlikely previous exposure to the virus. The innovative and integrative approach presented here to analyze the evolution of neutralizing antibodies from natural infection on a single-cell and repertoire level can also be applied to vaccine studies, and could potentially aid the development of therapeutic antibodies and our basic understanding of other infectious diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.