ArticleNucleic acids research2016
Synthesis and cell-free cloning of DNA libraries using programmable microfluidics.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Versioning biological cells for trustworthy cell engineering.Nature communications · 2022Article
- BiowareCFP: An Application-Agnostic Modular Reconfigurable Cyber-Fluidic Platform.Micromachines · 2022Article
- SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cell-free biology using remote-controlled digital microfluidics for individual droplet control.RSC advances · 2020Article
- Improving Reproducibility in Synthetic Biology.Frontiers in bioengineering and biotechnology · 2019Article
- Modular 5'-UTR hexamers for context-independent tuning of protein expression in eukaryotes.Nucleic acids research · 2018Article
- DNA assembly with error correction on a droplet digital microfluidics platform.BMC biotechnology · 2018Article
- Review
- Open-source, community-driven microfluidics with Metafluidics.Nature biotechnology · 2017Article
- The evolving art of creating genetic diversity: From directed evolution to synthetic biology.Biotechnology advancesReview
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, combinatorial assembly and cell-free cloning of genes, respectively. Proof-of-concept for these methods was demonstrated by programming an autonomous microfluidic system to construct and clone libraries of yeast ribosome binding sites and bacterial Azurine, which were then retrieved in individual droplets and validated. The ability to rapidly and robustly generate designer DNA molecules in an autonomous manner should have wide application in biological research and development.
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Registered trials
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