ArticleScientific reports2015
Protein array identification of protein markers for serodiagnosis of Mycobacterium tuberculosis infection.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection ofJournal of clinical microbiology · 2026Article
- The multiple roles of the NlpC_P60 peptidase family in mycobacteria - an underexplored target for antimicrobial drug discovery.FEBS letters · 2025Review
- Flotillin-mediated stabilization of unfolded proteins in bacterial membrane microdomains.Nature communications · 2024Article
- Article
- Evaluation of immunodominant peptides of in vivo expressed mycobacterial antigens in an ELISA-based diagnostic assay for pulmonary tuberculosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- The Rv3874-Rv3875 chimeric protein shows a promiscuous serodiagnostic potential for tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2022Article
- Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected withArchives of Razi Institute · 2022Article
- Advanced strategies for development of vaccines against human bacterial pathogens.World journal of microbiology & biotechnology · 2021Review
- Development and Evaluation of a Fusion Polyprotein Based on HspX and Other Antigen Sequences for the Serodiagnosis of Tuberculosis.Frontiers in immunology · 2021Article
- Comprehensive plasma proteomic profiling reveals biomarkers for active tuberculosis.JCI insight · 2020Article
- Characterization of antibody response against 16kD and 38kD ofAnnals of translational medicine · 2020Article
- An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation.PloS one · 2020Article
- Elevated circulating macrophage inhibitory cytokine 1 is a biological marker for the diagnosis and prognosis of osteosarcoma.Experimental and therapeutic medicine · 2018Article
- A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection.Journal of visualized experiments : JoVE · 2018Article
- Recent Trends in System-Scale Integrative Approaches for Discovering Protective Antigens Against Mycobacterial Pathogens.Frontiers in genetics · 2018Review
- Article
- Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection.PloS one · 2018Article
- ORFeome-based identification of biomarkers for serodiagnosis of Mycobacterium tuberculosis latent infection.BMC infectious diseases · 2017Article
- Secretome Prediction of TwoFrontiers in microbiology · 2017Article
- Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The lack of effective and accurate diagnostic tools contributes to the high prevalence of tuberculosis (TB) worldwide. The current serodiagnostics for TB are inadequate mainly due to lack of TB-specific antigens with highly accurate diagnosis. In the current study, we aimed to identify novel diagnostic antigens using glutathione S-transferase (GST)-fusion protein technique. We determined the reactivity of these recombinant proteins arrayed in solution and on GSH-immobilized microplates with TB patient sera. Of 409 TB proteins produced, ninety-two yielded seropositive reactions, fourteen including eight novel proteins showed strong immunoreactivity. Further, six were selected and constructed as a multiple-antigen combination set through analysis of various combinations. A comparative study of the multiple-antigen combination set and a commercially available kit revealed that the combination set showed 66.3% (95% CI 60.5-71.8) sensitivity, which was significantly higher than that of the commercial kit [31.6% (95% CI 26.3-37.3)]. The specificity of both methods was similar at 89.6% (95% CI 83.3-95.4) and 90.6% (95% CI 83.0-95.6), respectively. This study provides a set of novel diagnostic protein markers with great potential for the development of novel diagnostic tools for active TB.
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