ArticleTransfusion2016
CO2 -dependent metabolic modulation in red blood cells stored under anaerobic conditions.
Article in Transfusion, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 58 citations in OpenAlex.
- Hypoxic-hypocapnic red blood cells in PAGGSM additive solution before and after gamma irradiation show improved metabolism.Vox sanguinis · 2026Article
- Narrative review of strategies for blood product shelf-life extension.Transfusion · 2026Review
- Human red blood cell ATP content and export under hypoxic and/or isocapnic storage conditions.Frontiers in physiology · 2025Article
- Assessing the kinetics of oxygen-unloading from red cells using FlowScore, a flow-cytometric proxy of the functional quality of blood.EBioMedicine · 2025Article
- It's in your blood: The impact of age, sex, genetic factors and exposures on stored red blood cell metabolism.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2024Review
- Modulation of Antioxidant Enzyme Expression of In Vitro Culture-Derived Reticulocytes.Antioxidants (Basel, Switzerland) · 2024Article
- Article
- Atomic Force Microscopy and High-Resolution Spectrophotometry for Study of Anoxemia and Normoxemia in Model Experiment In Vitro.International journal of molecular sciences · 2023Article
- Red Blood Cell Metabolism In Vivo and In Vitro.Metabolites · 2023Review
- Sphingosine 1-phosphate has a negative effect on RBC storage quality.Blood advances · 2023Article
- Hypoxic storage of murine red blood cells improves energy metabolism and post-transfusion recoveries.Blood transfusion = Trasfusione del sangue · 2023Article
- Benefits of hypoxic storage of red blood cells.Blood transfusion = Trasfusione del sangue · 2023Article
- Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life.Nature communications · 2022Article
- Metabolic reprogramming under hypoxic storage preserves faster oxygen unloading from stored red blood cells.Blood advances · 2022Article
- Preclinical evaluation of the preservation of red blood cell concentrates by hypoxic storage technology for transfusion in sickle cell disease.Haematologica · 2022Article
- A deep 96-well plate RBC storage platform for high-throughput screening of novel storage solutions.Frontiers in physiology · 2022Article
- Effect of Hypoxic Blood Infusion on Pulmonary Physiology.Frontiers in physiology · 2022Review
- High-Throughput Metabolomics Platform for the Rapid Data-Driven Development of Novel Additive Solutions for Blood Storage.Frontiers in physiology · 2022Article
- Generation and Export of Red Blood Cell ATP in Health and Disease.Frontiers in physiology · 2021Review
- Impact of taurine on red blood cell metabolism and implications for blood storage.Transfusion · 2020Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundAnaerobic red blood cell (RBC) storage reduces oxidative damage, maintains adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (DPG) levels, and has superior 24-hour recovery at 6 weeks compared to standard storage. This study will determine if removal of CO2 during O2 depletion by gas exchange may affect RBCs during anaerobic storage. STUDY DESIGN AND
methodsThis is a matched three-arm study (n = 14): control, O2 and CO2 depleted with Ar (AN), and O2 depleted with 95%Ar/5%CO2 (AN[CO2 ]). RBCs in additives AS-3 or OFAS-3 were evenly divided into three bags, and anaerobic conditions were established by gas exchange. Bags were stored at 1 to 6°C in closed chambers under anaerobic conditions or ambient air, sampled weekly for up to 9 weeks for a panel of in vitro tests. A full metabolomics screening was conducted for the first 4 weeks of storage.
resultsPurging with Ar (AN) results in alkalization of the RBC and increased glucose consumption. The addition of 5% CO2 to the purging gas prevented CO2 loss with an equivalent starting and final pH and lactate to control bags (p > 0.5, Days 0-21). ATP levels are higher in AN[CO2 ] (p < 0.0001). DPG was maintained beyond 2 weeks in the AN arm (p < 0.0001). Surprisingly, DPG was lost at the same rate in both control and AN[CO2 ] arms (p = 0.6).
conclusionMaintenance of ATP in the AN[CO2 ] arm demonstrates that ATP production is not solely a function of the pH effect on glycolysis. CO2 in anaerobic storage prevented the maintenance of DPG, and DPG production appears to be pH dependent. CO2 as well as O2 depletion provides metabolic advantage for stored RBCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.