ArticleScientific reports2015
Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Neuromodulatory circuit effects on Drosophila feeding behaviour and metabolism.Scientific reports · 2017Pooled it
- Limited Evidence That Developmental Diet and Density Affect Adult Female Earwig Behaviour Despite Repeatable Individual Differences.Ecology and evolution · 2026Article
- A practical low-cost setup for anesthesia of the fruit flyJournal of microbiology & biology education · 2026Article
- Genetic heterogeneity alleviates behavioral trade-off between exploration and vigilance iniScience · 2026Article
- FLEX: Flight Exercise Training Protocol for the Fruit Fly Drosophila.Journal of visualized experiments : JoVE · 2025Article
- Multigenerational Effects of Weathered Polyethylene Microplastics onEnvironment & health (Washington, D.C.) · 2025Article
- Article
- Article
- Loss of the NF-κB negative regulator Pirk inBrain communications · 2025Article
- Article
- Novel Genome-EngineeredGenes · 2024Article
- Article
- Combined acid hydrolysis and fermentation improves bioactivity of citrus flavonoids in vitro and in vivo.Communications biology · 2023Article
- Indirect genetic effects for social network structure inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Imaginal disk growth factors are Drosophila chitinase-like proteins with roles in morphogenesis and CO2 response.Genetics · 2023Article
- Protocol for recording and analyzing spontaneous locomotion in Drosophila.STAR protocols · 2022Article
- Identification of evolutionarily conserved regulators of muscle mitochondrial network organization.Nature communications · 2022Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Glial immune-related pathways mediate effects of closed head traumatic brain injury on behavior and lethality in Drosophila.PLoS biology · 2022Article
- Excessive energy expenditure due to acute physical restraint disrupts Drosophila motivational feeding response.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laboratories that study Drosophila melanogaster or other insects commonly use carbon dioxide (CO2) anaesthesia for sorting or other work. Unfortunately, the use of CO2 has potential unwanted physiological effects, including altered respiratory and muscle physiology, which impact motor function behaviours. The effects of CO2 at different levels and exposure times were examined on the subsequent recovery of motor function as assessed by climbing and flight assays. With as little as a five minute exposure to 100% CO2, D. melanogaster exhibited climbing deficits up to 24 hours after exposure. Any exposure length over five minutes produced climbing deficits that lasted for days. Flight behaviour was also impaired following CO2 exposure. Overall, there was a positive correlation between CO2 exposure length and recovery time for both behaviours. Furthermore, exposure to as little as 65% CO2 affected the motor capability of D. melanogaster. These negative effects are due to both a CO2-specific mechanism and an anoxic effect. These results indicate a heretofore unconsidered impact of CO2 anaesthesia on subsequent behavioural tests revealing the importance of monitoring and accounting for CO2 exposure when performing physiological or behavioural studies in insects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.