ArticleApplied microbiology and biotechnology2016
Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1.
Article in Applied microbiology and biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- Co-expression, purification, and characterization of an acidophilic and n-hexane-tolerant lipase with its foldase from Burkholderia gladioli Bsp-1.Applied microbiology and biotechnology · 2026Article
- Comparative genomic analyses shed light on the introduction routes of rice-pathogenic Burkholderia gladioli strains into Bangladesh.BMC genomics · 2026Article
- Exploring the Interplay between Nutrients, Bacteriophages, and Bacterial Lipases in Host- and Bacteria-mediated Pathogenesis.Endocrine, metabolic & immune disorders drug targets · 2024Article
- Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay.Microbial cell factories · 2021Article
- Complete Genome Sequence of the NovelMicrobiology resource announcements · 2020Article
- A cleaner process of deinking waste paper pulp using Pseudomonas mendocina ED9 lipase supplemented enzyme cocktail.Environmental science and pollution research international · 2020Article
- Identification of lipolytic enzymes isolated from bacteria indigenous toBiotechnology reports (Amsterdam, Netherlands) · 2017Article
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Authors and funding
11 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia glumae is a Gram-negative phytopathogenic bacterium known as the causative agent of rice panicle blight. Strain B. glumae PG1 is used for the production of a biotechnologically relevant lipase, which is secreted into the culture supernatant via a type II secretion pathway. We have comparatively analyzed the genome sequences of B. glumae PG1 wild type and a lipase overproducing strain obtained by classical strain mutagenesis. Among a total number of 72 single nucleotide polymorphisms (SNPs) identified in the genome of the production strain, two were localized in front of the lipAB operon and were analyzed in detail. Both mutations contribute to a 100-fold overproduction of extracellular lipase in B. glumae PG1 by affecting transcription of the lipAB operon and efficiency of lipase secretion. We analyzed each of the two SNPs separately and observed a stronger influence of the promoter mutation than of the signal peptide modification but also a cumulative effect of both mutations. Furthermore, fusion of the mutated LipA signal peptide resulted in a 2-fold increase in secretion of the heterologous reporter alkaline phosphatase from Escherichia coli.
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