Evidence map›Paper›PMID 26476530›Full record

ArticleMolecular biology reports2015

SSR markers: a tool for species identification in Psidium (Myrtaceae).

A C Tuler, T T Carrijo, L R Nóia, A Ferreira, A L Peixoto, M F da Silva Ferreira

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Article in Molecular biology reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
5.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 87 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

A C Tuler
T T Carrijo
L R Nóia
A Ferreira
A L Peixoto
M F da Silva Ferreira
Universidade Federal do Espírito Santo · BRInstituto de Pesquisas Jardim Botânico do Rio de Janeiro · BRNational Tropical Botanical Garden · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Molecular DNA markers are used for detection of polymorphisms in individuals. As they are independent of developmental stage of the plant and environmental influences, they can be useful tools in taxonomy. The alleles of simple sequence repeat (SSR) markers (or microsatellites) are traditionally used to identify taxonomic units. This application demands the laborious and costly delimitation of exclusive alleles in order to avoid homoplasy. Here, we propose a method for identification of species based on the amplification profile of groups of SSR markers obtained by a transferability study. The approach considers that the SSR are conserved among related species. In this context, using Psidium as a model, 141 SSR markers developed for Psidium guajava were transferred to 13 indigenous species of Psidium from the Atlantic Rainforest. Transferability of the markers was high and 28 SSR were conserved in all species. Four SSR groups were defined and they can help in the identification of all 13 Psidium species studied. A group of 31 SSR was genotyped, with one to six alleles each. The H0 varied from 0.0 to 0.46, and PIC from 0.0 to 0.74. Cluster analysis revealed shared alleles among species. The high percentage of SSR transferability found in Psidium evidences the narrow phylogenetic relationship existing among these species since transferability occurs by the preservation of the microsatellites and anchoring regions. The proposed method was useful for distinguishing the species of Psidium, being useful in taxonomic studies.

Indexed as

Genome, PlantMicrosatellite RepeatsGenetic MarkersPhylogenyPolymorphism, GeneticPsidiumGenetic Markers

Identifiers

PMID26476530
OpenAlexW1954088307

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.