ArticleExtremophiles : life under extreme conditions2016
Cloning, expression and characterization of a novel cold‑adapted GDSL family esterase from Photobacterium sp. strain J15.
Article in Extremophiles : life under extreme conditions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 38 citations in OpenAlex.
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- A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp.Marine biotechnology (New York, N.Y.) · 2024Article
- X-ray crystallography of mutant GDSL esterase S12A of3 Biotech · 2023Article
- Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence.Current microbiology · 2022Article
- Cloning, Expression, and Purification of a GDSL-like Lipase/Acylhydrolase from a Native Lipase-Producing Bacterium, Lactobacillus fermentumIranian biomedical journal · 2022Article
- Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis.Frontiers in plant science · 2022Article
- Identification and Characterization of a Novel Thermostable GDSL-Type Lipase fromJournal of microbiology and biotechnology · 2021Article
- Elevating seed oil content in a polyploid crop by induced mutations in SEED FATTY ACID REDUCER genes.Plant biotechnology journal · 2020Article
- Green Synthesis of the Flavor Esters with a MarineIndian journal of microbiology · 2020Article
- Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases.Biotechnology for biofuels · 2020Article
- Biodiesel and flavor compound production using a novel promiscuous cold-adapted SGNH-type lipase (Biotechnology for biofuels · 2020Article
- Characterization and Immobilization of a Novel SGNH Family Esterase (International journal of molecular sciences · 2019Article
- Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization ofPeerJ · 2019Article
- Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7.Extremophiles : life under extreme conditions · 2017Article
- Complete Genome Sequence of Photobacterium sp. Strain J15, Isolated from Seawater of Southwestern Johor, Malaysia.Genome announcements · 2016Article
- Biochemical characterization and structural analysis of a new cold-active and salt-tolerant esterase from the marine bacterium Thalassospira sp.Extremophiles : life under extreme conditions · 2016Article
- Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes.Frontiers in microbiology · 2016Review
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The gene encoding for a novel cold-adapted enzyme from family II of bacterial classification (GDSL family) was cloned from the genomic DNA of Photobacterium sp. strain J15 in an Escherichia coli system, yielding a recombinant 36 kDa J15 GDSL esterase which was purified in two steps with a final yield and purification of 38.6 and 15.3 respectively. Characterization of the biochemical properties showed the J15 GDSL esterase had maximum activity at 20 °C and pH 8.0, was stable at 10 °C for 3 h and retained 50 % of its activity after a 6 h incubation at 10 °C. The enzyme was activated by Tween-20, -60 and Triton-X100 and inhibited by 1 mM Sodium dodecyl sulphate (SDS), while β-mercaptoethanol and Dithiothreitol (DTT) enhanced activity by 4.3 and 5.4 fold respectively. These results showed the J15 GDSL esterase was a novel cold-adapted enzyme from family II of lipolytic enzymes. A structural model constructed using autotransporter EstA from Pseudomonas aeruginosa as a template revealed the presence of a typical catalytic triad consisting of a serine, aspartate, and histidine which was verified with site directed mutagenesis on active serine.
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